Rabbit anti-Human DHX8 Polyclonal Antibody | anti-DHX8 antibody

DHX8 (ATP-Dependent RNA Helicase DHX8, DEAH box Protein 8, RNA Helicase HRH1, DHX8, DDX8) (HRP)

Cat. Num. AAA6455484

• Gene Names: DHX8; DDX8; HRH1; PRP22; PRPF22
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DHX8; Polyclonal Antibody; DHX8 (ATP-Dependent RNA Helicase DHX8; DEAH box Protein 8; RNA Helicase HRH1; DDX8) (HRP); anti-DHX8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DHX8. Species Crossreactivity: human
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DHX8 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH conjugated synthetic peptide corresponding to aa339-373 at the N-terminal region of human DHX8. Species sequence homology: mouse
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Product Categories/Family for anti-DHX8 antibody

Antibodies; DEAD Box Proteins

References

Ono Y., et al. Mol. Cell. Biol. 14:7611-7620(1994). Ohno M., et al. Genes Dev. 10:997-1007(1996). Jurica M.S., et al. RNA 8:426-439(2002). Neuhaus E.M., et al. J. Cell Sci. 119:3047-3056(2006). Dephoure N., et al. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4826690
• NCBI GeneID: 1659
• NCBI Accession #: NP_004932.1
• NCBI GenBank Nucleotide #: NM_004941.1
• UniProt Accession #: Q14562; A2A4P0
• Molecular Weight: 139,315 Da
• NCBI Official Full Name: ATP-dependent RNA helicase DHX8
• NCBI Official Synonym Full Names: DEAH (Asp-Glu-Ala-His) box polypeptide 8
• NCBI Official Symbol: DHX8
• NCBI Official Synonym Symbols: DDX8; HRH1; PRP22; PRPF22
• NCBI Protein Information: ATP-dependent RNA helicase DHX8; RNA helicase HRH1; DEAH box protein 8; DEAH-box protein 8; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase)
• UniProt Protein Name: ATP-dependent RNA helicase DHX8
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DHX8
• UniProt Synonym Gene Names: DDX8
• UniProt Entry Name: DHX8_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is highly homologous to yeast Prp22. This protein facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX8: Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.

Protein type: RNA-binding; RNA processing; RNA splicing; EC 3.6.4.13; Helicase; Spliceosome

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; ATP binding; ATP-dependent RNA helicase activity

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing