Rabbit anti-Human, Mouse DHX33 Polyclonal Antibody | anti-DHX33 antibody
DHX33 Antibody
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
DHX33; Polyclonal Antibody; DHX33 Antibody; DDX33; DEAD box polypeptide 33; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 33; DEAH (Asp Glu Ala His) box polypeptide 33; DEAH box protein 33; FLJ21972; anti-DHX33 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
DHX33 Antibody detects endogenous levels of DHX33.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-DHX33 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human DHX33.
Conjugation
Unconjugated
Similarity
Belongs to the DEAD box helicase family. DEAH subfamily.
Post Translational Modifications
Ubiquitinated, leading to its degradation by the proteasome. Deubiquitinated by USP36.
Subunit Structure
Interacts with UBTF. Interacts with DDX3X, EIF3G and EIF3H; the interaction is independent of RNA. Interacts (via HA2 region and Helicase C-terminal domain) with the components of the large ribosomal subunit RPL3, RPL7, RPL26 and RPL27. Interacts (via DEAH box) with NLRP3 (via NACHT domain). Binds to mRNA. Binds to double-stranded RNA (via the helicase C-terminal domain). Interacts (via the helicase C-terminal domain) with MAVS (By similarity).
Function
Implicated in nucleolar organization, ribosome biogenesis, protein synthesis and cytoplasmic dsRNA sensing (By similarity). Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment. In the cytoplasm, promotes elongation-competent 80S ribosome assembly at the late stage of mRNA translation initiation. Senses cytosolic dsRNA mediating NLRP3 inflammasome formation in macrophages and type I interferon production in myeloid dendritic cells. Required for NLRP3 activation induced by viral dsRNA and bacterial RNA. In dendritic cells, required for induction of type I interferon production induced by cytoplasmic dsRNA via the activation of MAPK and NF-kappa-B signaling pathways (By similarity).
Note
Predominantly in the nucleolus. During mitosis, localizes with the nucleolar organizing regions (PubMed:21930779). Upon dsRNA-binding, localizes in the inflammasome (PubMed:23871209).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-DHX33 antibody
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted Mol.Wt.: 79kDa(Calculated)
UniProt Protein Name
Putative ATP-dependent RNA helicase DHX33
Protein Family
UniProt Gene Name
DHX33
UniProt Synonym Gene Names
DDX33
UniProt Entry Name
DHX33_HUMAN
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Product Notes
The DHX33 dhx33 (Catalog #AAA9631007) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHX33 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DHX33 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the DHX33 dhx33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHX33, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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