Rabbit anti-Human, Mouse DHTKD1 Polyclonal Antibody | anti-DHTKD1 antibody

DHTKD1, ID (DHTKD1, KIAA1630, Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial, Dehydrogenase E1 and transketolase domain-containing protein 1) (PE)

Cat. Num. AAA6292090

• Gene Names: DHTKD1; CMT2Q; AMOXAD
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DHTKD1; Polyclonal Antibody; ID (DHTKD1; KIAA1630; Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1; mitochondrial; Dehydrogenase E1 and transketolase domain-containing protein 1) (PE); Dehydrogenase E1 and transketolase domain-containing protein 1; anti-DHTKD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DHTKD1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DHTKD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-425 amino acids from the Central region of human DHTKD1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DHTKD1 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the DHTKD1 antibody detected the DHTKD1 protein (arrow).)

Product Categories/Family for anti-DHTKD1 antibody

Antibodies; Mitochondrial Proteins

References

Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006). Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 38788380
• NCBI GeneID: 55526
• NCBI Accession #: NP_061176.3
• NCBI GenBank Nucleotide #: NM_018706.6
• UniProt Accession #: Q96HY7; Q68CU5; Q9BUM8; Q9HCE2
• Molecular Weight: 103,077 Da
• NCBI Official Full Name: probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
• NCBI Official Synonym Full Names: dehydrogenase E1 and transketolase domain containing 1
• NCBI Official Symbol: DHTKD1
• NCBI Official Synonym Symbols: CMT2Q; AMOXAD
• NCBI Protein Information: probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
• UniProt Protein Name: Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
• Protein Family: Probable 2-oxoglutarate dehydrogenase E1 component
• UniProt Gene Name: DHTKD1
• UniProt Synonym Gene Names: KIAA1630

NCBI Description

This gene encodes a component of a mitochondrial 2-oxoglutarate-dehydrogenase-complex-like protein involved in the degradation pathways of several amino acids, including lysine. Mutations in this gene are associated with 2-aminoadipic 2-oxoadipic aciduria and Charcot-Marie-Tooth Disease Type 2Q. [provided by RefSeq, May 2013]

Uniprot Description

DHTKD1: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). Belongs to the alpha-ketoglutarate dehydrogenase family.

Protein type: EC 1.2.4.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 10p14

Cellular Component: cytosol; mitochondrial matrix; mitochondrion; oxoglutarate dehydrogenase complex

Molecular Function: oxoglutarate dehydrogenase (succinyl-transferring) activity; thiamine pyrophosphate binding

Biological Process: generation of precursor metabolites and energy; glycolytic process; hemopoietic progenitor cell differentiation; tricarboxylic acid cycle

Disease: 2-aminoadipic 2-oxoadipic Aciduria; Charcot-marie-tooth Disease, Axonal, Type 2q