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Western Blot (WB) (Western blot analysis of DHRS9 using HepG2 whole cell lysates)

Rabbit DHRS9 Polyclonal Antibody | anti-DHRS9 antibody

DHRS9 Antibody

Gene Names
DHRS9; RDHL; RDH15; RDH-E2; RDHTBE; SDR9C4; RDH-TBE; RETSDR8; 3ALPHA-HSD; 3-alpha-HSD
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DHRS9; Polyclonal Antibody; DHRS9 Antibody; 3 alpha hydroxysteroid dehydrogenase; 3-alpha-HSD; 3-alpha-hydroxysteroid dehydrogenase; 3-alpha hydroxysteroid dehydrogenase; 3alpha HSD; Dehydrogenase/reductase SDR family member 9; DHRS9_HUMAN; NADP dependent retinol dehydrogenase/reductase; NADP-dependent retinol dehydrogenase/reductase; RDH-E2; Rdh15; RDHE2; RDHL; RDHTBE; retinol dehydrogenase homolog; Retinol dehydrogenase; tracheobronchiol epithelail cell-specific; RETSDR8; SDR family; member 9; SDR9C4; short chain dehydrogenase/reductase family 9; short chain dehydrogenase/reductase family 9C; member 4; Short chain dehydrogenase/reductase retSDR8; Short-chain dehydrogenase/reductase retSDR8; anti-DHRS9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DHRS9 antibody detects endogenous levels of total DHRS9
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
319
Applicable Applications for anti-DHRS9 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
Immunogen
A synthesized peptide derived from human DHRS9
Subcellular Location
Microsome Membrane. Endoplasmic Reticulum Membrane. Associated with microsomal membranes.
Tissue Specificity
Highly expressed in trachea and epidermis. Detected at lower levels in spinal cord, bone marrow, brain, tongue, esophagus, heart, colon, testis, placenta, lung, skeletal muscle and lymph node.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of DHRS9 using HepG2 whole cell lysates)

Western Blot (WB) (Western blot analysis of DHRS9 using HepG2 whole cell lysates)

Immunohistochemistry (IHC)

(MBS9609755 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9609755 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-DHRS9 antibody
Function: 3-alpha-hydroxysteroid dehydrogenase that converts 3-alpha-tetrahydroprogesterone (allopregnanolone) to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone. May play a role in the biosynthesis of retinoic acid from retinaldehyde, but seems to have low activity with retinoids. Can utilize both NADH and NADPH.
Subunit Structure: Homotetramer.
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 35 kDa
Predicted: 36 kDa
NCBI Official Full Name
dehydrogenase/reductase SDR family member 9 isoform 1
NCBI Official Synonym Full Names
dehydrogenase/reductase 9
NCBI Official Symbol
DHRS9
NCBI Official Synonym Symbols
RDHL; RDH15; RDH-E2; RDHTBE; SDR9C4; RDH-TBE; RETSDR8; 3ALPHA-HSD; 3-alpha-HSD
NCBI Protein Information
dehydrogenase/reductase SDR family member 9
UniProt Protein Name
Dehydrogenase/reductase SDR family member 9
UniProt Gene Name
DHRS9
UniProt Synonym Gene Names
RDH15; SDR9C4; 3-alpha-HSD; RDH-TBE

NCBI Description

This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

3-alpha-hydroxysteroid dehydrogenase that converts 3-alpha-tetrahydroprogesterone (allopregnanolone) to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone. May play a role in the biosynthesis of retinoic acid from retinaldehyde, but seems to have low activity with retinoids. Can utilize both NADH and NADPH.

Research Articles on DHRS9

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Product Notes

The DHRS9 dhrs9 (Catalog #AAA9609755) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHRS9 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DHRS9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the DHRS9 dhrs9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHRS9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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