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Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using DHRS4 antibody.)

Rabbit anti-Human, Mouse DHRS4 Polyclonal Antibody | anti-DHRS4 antibody

DHRS4 Antibody

Gene Names
DHRS4; CR; NRDR; PHCR; PSCD; SDR-SRL; SDR25C1; SDR25C2; SCAD-SRL
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DHRS4; Polyclonal Antibody; DHRS4 Antibody; AI043103; AI790593; Carbonyl reductase; CR; D14Ucla2; Dehydrogenase/reductase SDR family member 4; Dhrs4; DHRS4_HUMAN; humNRDR; mouNRDR; NADPH dependent carbonyl reductase/NADP retinol dehydrogenase; NADPH dependent retinol dehydrogenase/reductase; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; NADPH-dependent retinol dehydrogenase/reductase; NDRD; NRDR; Peroxisomal short chain alcohol dehydrogenase; Peroxisomal short-chain alcohol dehydrogenase; PHCR; PRO1800; PSCD; RRD; SCAD-SRL; SCADSRL; Short chain dehydrogenase/reductase family member 4; Short-chain dehydrogenase/reductase family member 4; UNQ851; anti-DHRS4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
DHRS4 antibody detects endogenous levels of total DHRS4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
188
Applicable Applications for anti-DHRS4 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Peroxisome; Nucleus
Tissue Specificity
Isoform 1 is predominantly expressed in normal cervix (at protein level). Isoform 4 is expressed in some neoplastic cervical tissues, but not in normal cervix (at protein level). Isoform 5 and isoform 6 are expressed in a few neoplastic cervical tissues.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using DHRS4 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using DHRS4 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from Rat brain lysate, using DHRS4 antibody.)

Western Blot (WB) (Western blot analysis of extracts from Rat brain lysate, using DHRS4 antibody.)

Immunofluorescence (IF)

(MBS9605442 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Immunofluorescence (IF) (MBS9605442 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Immunohistochemistry (IHC)

(MBS9605442 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9605442 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-DHRS4 antibody
Function: Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones (By similarity).
Subunit Structure: Homotetramer.
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 32-34 kD
Predicted: 30 kDa
NCBI Official Full Name
dehydrogenase/reductase SDR family member 4 isoform 2
NCBI Official Synonym Full Names
dehydrogenase/reductase 4
NCBI Official Symbol
DHRS4
NCBI Official Synonym Symbols
CR; NRDR; PHCR; PSCD; SDR-SRL; SDR25C1; SDR25C2; SCAD-SRL
NCBI Protein Information
dehydrogenase/reductase SDR family member 4
UniProt Protein Name
Dehydrogenase/reductase SDR family member 4
UniProt Gene Name
DHRS4
UniProt Synonym Gene Names
SDR25C2; CR; PHCR; NRDR; humNRDR; PSCD

Uniprot Description

Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones ().

Research Articles on DHRS4

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Product Notes

The DHRS4 dhrs4 (Catalog #AAA9605442) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHRS4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DHRS4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the DHRS4 dhrs4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHRS4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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