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Immunofluorescence (IF) (Immunofluorescence staining of A549 cells with CSB-PA006851LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Rabbit anti-Human DHH Polyclonal Antibody | anti-DHH antibody

DHH Antibody

Gene Names
DHH; GDXYM; HHG-3; SRXY7
Reactivity
Human
Applications
ELISA, Immunofluorescence
Purity
>95%
Protein G Purified
Synonyms
DHH; Polyclonal Antibody; DHH Antibody; Desert hedgehog protein; HHG-3; [Cleaved into: Desert hedgehog protein N-product; Desert hedgehog protein C-product]; anti-DHH antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%
Protein G Purified
Form/Format
Liquid
Sequence Length
396
Applicable Applications for anti-DHH antibody
ELISA (EIA), Immunofluorescence (IF)
Application Notes
IF: 1:50-1:200
Immunogen Species
Human
Immunogen
Recombinant Human Desert hedgehog protein (51-158AA)
Conjugate
Non-Conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Immunofluorescence (IF)

(Immunofluorescence staining of A549 cells with CSB-PA006851LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Immunofluorescence (IF) (Immunofluorescence staining of A549 cells with CSB-PA006851LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Product Categories/Family for anti-DHH antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,577 Da
NCBI Official Full Name
desert hedgehog protein preproprotein
NCBI Official Synonym Full Names
desert hedgehog signaling molecule
NCBI Official Symbol
DHH
NCBI Official Synonym Symbols
GDXYM; HHG-3; SRXY7
NCBI Protein Information
desert hedgehog protein
UniProt Protein Name
Desert hedgehog protein
Protein Family
UniProt Gene Name
DHH
UniProt Synonym Gene Names
DHH

NCBI Description

This gene encodes a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq, May 2010]

Uniprot Description

Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

Research Articles on DHH

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Product Notes

The DHH dhh (Catalog #AAA7112912) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHH Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DHH can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF). IF: 1:50-1:200. Researchers should empirically determine the suitability of the DHH dhh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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