Rabbit anti-Mouse DHFR Polyclonal Antibody | anti-DHFR antibody

DHFR, CT (Dihydrofolate Reductase) (FITC)

Cat. Num. AAA6472760

• Gene Names: Dhfr; AA607882; AI662710; AW555094; 8430436I03Rik
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: DHFR; Polyclonal Antibody; CT (Dihydrofolate Reductase) (FITC); anti-DHFR antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse DHFR.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DHFR antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of mouse DHFR (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of DHFR antibody (C-term) in mouse liver tissue lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(DHFR Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHFR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of DHFR (arrow) using rabbit polyclonal DHFR Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHFR gene.)

Product Categories/Family for anti-DHFR antibody

Antibodies; Enzymes; Reductase

References

1. Gemmati, D., Am. J. Hematol. 84(8), 526-529 (2009). 2. Stockman, B.J., Biochemistry 31(1), 218-229 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 7106289
• NCBI GeneID: 13361
• NCBI Accession #: NP_034179
• NCBI GenBank Nucleotide #: NM_010049.3
• UniProt Accession #: P00375; P70693; Q61485; Q61487; Q61579
• Molecular Weight: 23.8 kDa (207aa), confirmed by MALDI-TOF
• NCBI Official Full Name: dihydrofolate reductase
• NCBI Official Synonym Full Names: dihydrofolate reductase
• NCBI Official Symbol: Dhfr
• NCBI Official Synonym Symbols: AA607882; AI662710; AW555094; 8430436I03Rik
• NCBI Protein Information: dihydrofolate reductase
• UniProt Protein Name: Dihydrofolate reductase
• Protein Family: Viral dihydrofolate reductase
• UniProt Gene Name: Dhfr
• UniProt Entry Name: DYR_MOUSE

Uniprot Description

DHFR: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD). DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. Belongs to the dihydrofolate reductase family.

Protein type: Cofactor and Vitamin Metabolism - folate biosynthesis; Oxidoreductase; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 1.5.1.3

Molecular Function: mRNA binding; dihydrofolic acid binding; RNA binding; oxidoreductase activity; drug binding; dihydrofolate reductase activity; NADP binding

Biological Process: tetrahydrofolate metabolic process; tetrahydrofolate biosynthetic process; nucleotide biosynthetic process; dihydrofolate metabolic process; one-carbon compound metabolic process; response to methotrexate; glycine biosynthetic process