Western Blot (WB)
(Western blot analysis of lysates from K562 cells, using DGKE Antibody. The lane on the right is blocked with the synthesized peptide.)
Rabbit DGKE Polyclonal Antibody | anti-DGKE antibody
DGKE Antibody
Gene Names
DGKE; DGK; AHUS7; DAGK5; DAGK6; NPHS7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
DGKE; Polyclonal Antibody; DGKE Antibody; Diacylglycerol kinase epsilon; Diglyceride kinase epsilon; DGK-epsilon; DAG kinase epsilon; DAGK5; anti-DGKE antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
DGKE Antibody detects endogenous levels of total DGKE protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
177
Applicable Applications for anti-DGKE antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:5000
ELISA: 1:5000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human DGKE.
Immunogen Range
161-210
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Western Blot (WB)
(Western blot analysis of lysates from K562 cells, using DGKE Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from K562 cells, using DGKE Antibody. The lane on the right is blocked with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63 kDa
NCBI Official Full Name
diacylglycerol kinase epsilon
NCBI Official Synonym Full Names
diacylglycerol kinase epsilon
NCBI Official Symbol
DGKE
NCBI Official Synonym Symbols
DGK; AHUS7; DAGK5; DAGK6; NPHS7
NCBI Protein Information
diacylglycerol kinase epsilon
UniProt Protein Name
Diacylglycerol kinase epsilon
Protein Family
UniProt Gene Name
DGKE
UniProt Synonym Gene Names
DAGK5; DAG kinase epsilon; DGK-epsilon
NCBI Description
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008]
Uniprot Description
Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Research Articles on DGKE
1. mutations can lead to atypical hemolytic uremic syndrome or membranoproliferative glomerulonephritis
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Product Notes
The DGKE dgke (Catalog #AAA9505102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DGKE Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DGKE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:5000. Researchers should empirically determine the suitability of the DGKE dgke for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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