Rabbit anti-Human DGCR8 Polyclonal Antibody | anti-DGCR8 antibody

DGCR8, ID (DiGeorge Syndrome Critical Region 8, Microprocessor Complex Subunit DGCR8, C22orf12, DGCRK6, LP4941)

Cat. Num. AAA625732

• Gene Names: DGCR8; Gy1; pasha; DGCRK6; C22orf12
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: DGCR8; Polyclonal Antibody; ID (DiGeorge Syndrome Critical Region 8; Microprocessor Complex Subunit DGCR8; C22orf12; DGCRK6; LP4941); Anti -DGCR8; anti-DGCR8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DGCR8.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-DGCR8 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the Center region of human DGCR8 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DGCR8 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). DGCR8 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(DGCR8 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DGCR8 antibody

MicroRNAs (miRNA) are a recently discovered family of short non-protein-coding RNAs that negatively regulate gene expression. Recent studies of miRNAs highlight a requirement for cell viability. Post-transcriptional silencing of target genes by miRNAs occurs either by targeting specific cleavage of homologous mRNAs, or by targeting specific inhibition of protein synthesis.

Product Categories/Family for anti-DGCR8 antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 8980662
• NCBI GeneID: 54487
• UniProt Accession #: Q53GY5; Q8WYQ5
• Molecular Weight: 64,487 Da
• NCBI Official Full Name: DGCR8
• NCBI Official Synonym Full Names: DiGeorge syndrome critical region gene 8
• NCBI Official Symbol: DGCR8
• NCBI Official Synonym Symbols: Gy1; pasha; DGCRK6; C22orf12
• NCBI Protein Information: microprocessor complex subunit DGCR8; OTTHUMP00000197673; OTTHUMP00000197674; DiGeorge syndrome critical region 8
• UniProt Protein Name: DiGeorge syndrome critical region gene 8 variant
• Protein Family: Microprocessor complex
• UniProt Entry Name: Q53GY5_HUMAN

NCBI Description

This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

DGCR8: a double strand RNA binding protein (dsRBP) which is essential for the first processing step of primary miRNAs (pri-miRNAs). Apparently binds and is activated by heme. A nuclear complex of Drosha with DGCR8 cleaves pri-miRNA substrates into 60-70 nucleotide intermediates (pre-miRNA), thus initiating the microRNA maturation process. Drosha provides the catalytic site for this cleavage, whereas DGCR8 (Pasha) anchors substrate pri-miRNAs. Pre-miRNAs are then processed by the cytoplasmic Dicer-TRBP complex to approximately 20-22 nt mature miRNAs.

Protein type: RNA-binding; RNA processing; Nucleolus

Chromosomal Location of Human Ortholog: 22q11.2

Cellular Component: nucleoplasm; microtubule cytoskeleton; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; ribonuclease III activity; protein homodimerization activity; metal ion binding; double-stranded RNA binding

Biological Process: gene expression; primary microRNA processing

Disease: Velocardiofacial Syndrome