Rabbit DFFA Polyclonal Antibody | anti-DFFA antibody

DFFA Polyclonal Antibody

Cat. Num. AAA3008356

• Gene Names: DFFA; DFF1; ICAD; DFF-45
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DFFA; Polyclonal Antibody; DFFA Polyclonal Antibody; DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD; DFF1; DFF45; H13; anti-DFFA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DFFA polyclonal antibody detects endogenous levels of DFFA protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 331

• Applicable Applications for anti-DFFA antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:100
• Immunogen: Recombinant protein of human DFFA.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using DFFA antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using DFFA antibody at dilution of 1:100 (x400 lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using DFFA antibody. Blue: DAPI for nuclear staining.)

Western Blot (WB)

(Western Blot analysis of extracts from normal (control) and DFFA knockout (KO) HeLa cells, using DFFA antibody.)

Related Product Information for anti-DFFA antibody

The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.

NCBI and Uniprot Product Information

• NCBI GI #: 4758148
• NCBI GeneID: 1676
• NCBI Accession #: NP_004392.1
• NCBI GenBank Nucleotide #: NP_004392.1
• UniProt Accession #: O00273
• Molecular Weight: ~ 35, 45kDa
• NCBI Official Full Name: DNA fragmentation factor subunit alpha isoform 1
• NCBI Official Synonym Full Names: DNA fragmentation factor subunit alpha
• NCBI Official Symbol: DFFA
• NCBI Official Synonym Symbols: DFF1; ICAD; DFF-45
• NCBI Protein Information: DNA fragmentation factor subunit alpha
• UniProt Protein Name: DNA fragmentation factor subunit alpha
• Protein Family: DNA fragmentation factor
• UniProt Gene Name: DFFA
• UniProt Synonym Gene Names: DFF1; DFF45; DFF-45; ICAD
• UniProt Entry Name: DFFA_HUMAN

NCBI Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase

Chromosomal Location of Human Ortholog: 1p36.3-p36.2

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; lipid particle; nucleus; cytosol

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis