Western Blot (WB)
(Anti-Desmoglein-2 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:122 kDa)
Rabbit anti-Human Desmoglein-2 Polyclonal Antibody | anti-DSG2 antibody
Anti-Desmoglein-2 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
Desmoglein-2; Polyclonal Antibody; Anti-Desmoglein-2 Antibody; Rabbit Polyclonal; Desmoglein-2/DSG2 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-ARVC10 Antibody; Anti-ARVD10 Antibody; Anti-CDHF5 Antibody; Anti-CMD1BB Antibody; Anti-HDGC Antibody; desmoglein 2; anti-DSG2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Desmoglein-2/DSG2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-DSG2 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-5uL/mg of lysate
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human Desmoglein-2/DSG2 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human Desmoglein-2/DSG2 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Western Blot (WB)
(Anti-Desmoglein-2 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:122 kDa)
Western Blot (WB)
(Anti-Desmoglein-2 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:122 kDa)
Immunoprecipitation (IP)
(DSG2 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-DSG2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DSG2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 122 kDa Observed band size :171 kDa)
Immunoprecipitation (IP)
(DSG2 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-DSG2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DSG2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 122 kDa Observed band size :171 kDa)
Related Product Information for anti-DSG2 antibody
Background: Desmoglein-2 (Dsg2) is a specific cadherin of the cell-cell contact in cardiac desmosomes. Mutations in the DSG2-gene are regarded to cause arrhythmogenic (right ventricular) cardiomyopathy (ARVC) which is a rare but severe heart muscle disease. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of beta-catenin/Slug-mediated epithelial-to-mesenchymal transition. Which demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.
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Product Notes
The DSG2 (Catalog #AAA8119420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Desmoglein-2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Desmoglein-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the DSG2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Desmoglein-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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