rabbit anti-Human Deoxyribonuclease II Polyclonal Antibody | anti-DNASE2 antibody

Deoxyribonuclease II (DNase II)

Cat. Num. AAA618695

• Gene Names: DNASE2; DNL; DNL2; DNASE2A
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Deoxyribonuclease II; Polyclonal Antibody; Deoxyribonuclease II (DNase II); Anti -Deoxyribonuclease II (DNase II); anti-DNASE2 antibody

• Host: rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.

• Applicable Applications for anti-DNASE2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:500-1:1000; Immunohistochemistry: 5ug/ml
• Immunogen: Synthetic peptide, KLH-conjugated. Epitope: peptide corresponding to amino acids 347 to 360 of human DNase II precursor (2-4)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Human Thymus (formalin-fixed, paraffin-embedded) stained with DNASE2 antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with DNASE2 antibody LS-B560 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.)

Product Categories/Family for anti-DNASE2 antibody

Antibodies; Abs to Enzymes; Deoxyribonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 14275880
• NCBI GeneID: 1777
• UniProt Accession #: O00115; O43910; B2RD06
• Molecular Weight: 39,581 Da
• NCBI Official Full Name: deoxyribonuclease II
• NCBI Official Synonym Full Names: deoxyribonuclease II, lysosomal
• NCBI Official Symbol: DNASE2
• NCBI Official Synonym Symbols: DNL; DNL2; DNASE2A
• NCBI Protein Information: deoxyribonuclease-2-alpha; R31240_2; acid DNase; DNase II alpha; lysosomal DNase II; DNase II, lysosomal; deoxyribonuclease II alpha
• UniProt Protein Name: Deoxyribonuclease-2-alpha
• Protein Family: Deoxyribonuclease
• UniProt Gene Name: DNASE2
• UniProt Synonym Gene Names: DNASE2A; DNL2
• UniProt Entry Name: DNS2A_HUMAN

NCBI Description

This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq]

Uniprot Description

DNASE2: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. Belongs to the DNase II family.

Protein type: EC 3.1.22.1; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome; intracellular

Molecular Function: DNA binding; deoxyribonuclease II activity

Biological Process: erythrocyte differentiation; DNA fragmentation during apoptosis; DNA metabolic process