Rabbit delta1-Catenin Polyclonal Antibody | anti-CTNND1 antibody

delta1-Catenin (Thr-916), phospho-specific Antibody

Cat. Num. AAA474287

• Gene Names: CTNND1; CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunocytochemistry
• Synonyms: delta1-Catenin; Polyclonal Antibody; delta1-Catenin (Thr-916); phospho-specific Antibody; phospho-specific; Catenin 4 916; anti-CTNND1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using phospho-delta1-Catenin (Thr-916) peptide (without carrier). The antibody detects a 110kDa* protein corresponding to the molecular mass of delta1-Catenin on SDS-PAGE immunoblots of A431 cells treated with calyculin A, but does not detect this band after lambda phosphatase treatment.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-CTNND1 antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; ICC: 1:50; WB: 1:1000
• Immunogen: Phospho-delta1-Catenin (Thr-916) synthetic peptide corresponds to amino acid residues around threonine 916 of human delta1-Catenin. This peptide sequence is highly conserved in rat and mouse delta1-Catenin.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of delta1-Catenin phosphorylation in A431 cells stimulated with calyculin A (100nM) for 30 min. (lanes 1 & 3) then the blot was treated with lambda phosphatase (lanes 2 & 4). The blots were probed with either mouse monoclonal anti-delta1-Catenin (a.a. 275-285) (lanes 1 & 2) or rabbit polyclonal anti-delta1-Catenin (Thr-916) (lanes 3 & 4).)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of delta1-Catenin in control (Top row) or calyculin A-treated A431 cells (Bottom row). The cells were labeled with rabbit polyclonal delta1-Catenin (Thr-916) (Left) or mouse monoclonal delta1-Catenin (a.a. 275-285) (Middle). Demonstration of the overlapping labeling patterns is shown on the right. The antibodies were detected using goat anti-rabbit DyLight 488 or goat anti-mouse-DyLight 594.)

Related Product Information for anti-CTNND1 antibody

Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. delta1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. delta1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by delta1-Catenin, which can modulate RhoA, Rac and cdc42 activity. delta1-Catenin is phosphorylated at multiple tyrosine, serine and threonine sites both in vitro and in vivo. High levels of delta1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in delta1-Catenin associated with the plasma membrane.

References

Fukumoto, Y. et al. (2008) Exp. Cell Res. 314:52.
Reynolds, A.B. & Roczniak-Ferguson, A. (2004) Oncogene 23:7947.
Mariner, D.J. et al. (2001) J. Biol. Chem. 276:28006.

NCBI and Uniprot Product Information

• NCBI GI #: 146231940
• NCBI GeneID: 1500
• NCBI Accession #: NP_001078927.1
• NCBI GenBank Nucleotide #: NM_001085458.1
• UniProt Accession #: O60716; O15088; O60713; O60714; O60715; O60935; Q6DHZ7; Q6RBX8; Q9UP71; Q9UP72; Q9UP73; A8K939
• Molecular Weight: 108,170 Da
• NCBI Official Full Name: catenin delta-1 isoform 1ABC
• NCBI Official Synonym Full Names: catenin (cadherin-associated protein), delta 1
• NCBI Official Symbol: CTNND1
• NCBI Official Synonym Symbols: CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
• NCBI Protein Information: catenin delta-1; p120 catenin; cadherin-associated Src substrate
• UniProt Protein Name: Catenin delta-1
• Protein Family: Catenin
• UniProt Gene Name: CTNND1
• UniProt Synonym Gene Names: KIAA0384; CAS; p120(ctn)
• UniProt Entry Name: CTND1_HUMAN

NCBI Description

This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

CTNND1: an Arm repeat protein that interacts with varied components such as cadherin, small G proteins, kinases, and the Kaiso transcriptional repressor. A downstream modulator of Wnt/beta-catenin signaling pathway whose stability may be regulated downstream of Dsh. Required for the invasiveness of E-cadherin-deficient cells. A signal coordinator between cadherins and Rho-family GTPases to regulate cytoskeletal changes required during dendritic spine and synapse development. The association of catenins with cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Thirty two splice-variant isoforms have been described.

Protein type: Adaptor/scaffold; G protein regulator, misc.; Motility/polarity/chemotaxis; Cell adhesion; Actin-binding

Chromosomal Location of Human Ortholog: 11q11

Cellular Component: growth cone; lamellipodium; cytoplasm; dendritic spine; plasma membrane; synapse; intercellular junction; midbody; zonula adherens; nucleus; cytosol

Molecular Function: protein binding; cadherin binding; protein kinase binding; receptor binding

Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; Wnt receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; brain development; vascular endothelial growth factor receptor signaling pathway; cell adhesion