Western Blot (WB)
(Western blot analysis of Mouse liver lysate, using DEFB132 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit DEFB132 Polyclonal Antibody | anti-DEFB132 antibody
DEFB132 Antibody
Gene Names
DEFB132; BD-32; DEFB32; HEL-75; UNQ827; DEFB-32; KFLL827
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DEFB132; Polyclonal Antibody; DEFB132 Antibody; BD-32; beta 132; Beta-defensin 132; Beta-defensin 32; DB132_HUMAN; DEFB-32; Defensin; Defensin HEL-75; UNQ827; anti-DEFB132 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DEFB132 antibody detects endogenous levels of total DEFB132
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
95
Applicable Applications for anti-DEFB132 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human DEFB132
Subcellular Location
Secreted.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Mouse liver lysate, using DEFB132 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of Mouse liver lysate, using DEFB132 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9606878 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9606878 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-DEFB132 antibody
Description: Beta-defensins (also designated BDs, or hBDs in human) are small cationic peptides with broad-spectrum antimicrobial activity against a variety of enveloped viruses, fungi and bacteria. Produced in mucosal epithelia and neutrophils of several species, beta-defensins are developmentally regulated. The family of beta-defensin proteins share a common defensin-motif that is characterized by multiple cysteine residues and a highly conserved tertiary structure. Besides playing a significant role in host immune defense, many beta-defensins also are involved in sperm maturation and capacitation. beta-defensin 32, also known as beta-defensin 132, is a 95 amino acid secreted protein that most likely contains a signal peptide sequence that requires cleavage by proteolytic enzymes in order to become biologically active.
Function: Has antibacterial activity.
Similarity: Belongs to the beta-defensin family.
Function: Has antibacterial activity.
Similarity: Belongs to the beta-defensin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 11 kDa
Predicted: 11 kDa
Predicted: 11 kDa
NCBI Official Full Name
beta-defensin 132
NCBI Official Synonym Full Names
defensin beta 132
NCBI Official Symbol
DEFB132
NCBI Official Synonym Symbols
BD-32; DEFB32; HEL-75; UNQ827; DEFB-32; KFLL827
NCBI Protein Information
beta-defensin 132
UniProt Protein Name
Beta-defensin 132
Protein Family
UniProt Gene Name
DEFB132
UniProt Synonym Gene Names
DEFB32; BD-32; DEFB-32
NCBI Description
Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The protein encoded by this gene is secreted and is a member of the beta defensin protein family. This protein binds spermatozoa and has antimicrobial activity against E. coli. Beta defensin genes are found in several clusters throughout the genome, with this gene mapping to a cluster at 20p13. [provided by RefSeq, Nov 2014]
Uniprot Description
Has antibacterial activity.
Research Articles on DEFB132
Similar Products
Product Notes
The DEFB132 defb132 (Catalog #AAA9606878) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DEFB132 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DEFB132 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the DEFB132 defb132 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEFB132, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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