Western Blot (WB)
(Western blot analysis of DEDD using K562 whole cell lysates)
Rabbit DEDD Polyclonal Antibody | anti-DEDD antibody
DEDD Antibody
Gene Names
DEDD; DEFT; KE05; DEDD1; FLDED1; CASP8IP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DEDD; Polyclonal Antibody; DEDD Antibody; CASP8IP1; Death effector domain containing; Death effector domain containing protein; Death effector domain containing testicular molecule; Death effector domain-containing protein; Death effector domain-containing testicular molecule; DEDD 1; dedD; DEDD_HUMAN; DEDD1; DEDPro 1; DEDPro1; DEFT; FLDED 1; FLDED-1; FLDED1; KE 05; KE05; OTTHUMP00000031822; anti-DEDD antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DEDD antibody detects endogenous levels of total DEDD
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
318
Applicable Applications for anti-DEDD antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human DEDD
Subcellular Location
Cytoplasm. Nucleus > Nucleolus. Translocated to the nucleus during CD95-mediated apoptosis where it is localized in the nucleoli (By similarity). Following apoptosis induction, the mono and/or diubiquitination form increases and forms filamentous structures that colocalize with KRT8 and KRT18 intermediate filament network in simple epithelial cells.
Tissue Specificity
Widely expressed with highest levels in testis.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of DEDD using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis of DEDD using K562 whole cell lysates)
Related Product Information for anti-DEDD antibody
Function: A scaffold protein that directs CASP3 to certain substrates and facilitates their ordered degradation during apoptosis. May also play a role in mediating CASP3 cleavage of KRT18. Regulates degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3. Inhibits DNA transcription in vitro (By similarity).
Subunit Structure: Interacts with CASP8, CASP10, KRT8, KRT18, CASP3 and FADD. Homodimerizes and heterodimerizes with DEDD2.
Post-translational Modifications: Exists predominantly in a mono-or diubiquitinated form.
Subunit Structure: Interacts with CASP8, CASP10, KRT8, KRT18, CASP3 and FADD. Homodimerizes and heterodimerizes with DEDD2.
Post-translational Modifications: Exists predominantly in a mono-or diubiquitinated form.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 37 kDa
Predicted: 37 kDa
Predicted: 37 kDa
NCBI Official Full Name
death effector domain-containing protein isoform b
NCBI Official Synonym Full Names
death effector domain containing
NCBI Official Symbol
DEDD
NCBI Official Synonym Symbols
DEFT; KE05; DEDD1; FLDED1; CASP8IP1
NCBI Protein Information
death effector domain-containing protein
UniProt Protein Name
Death effector domain-containing protein
Protein Family
UniProt Gene Name
DEDD
UniProt Synonym Gene Names
DEDPRO1; DEFT
NCBI Description
This gene encodes a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus. At least three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
A scaffold protein that directs CASP3 to certain substrates and facilitates their ordered degradation during apoptosis. May also play a role in mediating CASP3 cleavage of KRT18. Regulates degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3. Inhibits DNA transcription in vitro ().
Research Articles on DEDD
Similar Products
Product Notes
The DEDD dedd (Catalog #AAA9602959) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DEDD Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DEDD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the DEDD dedd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEDD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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