Rabbit anti-Human, Mouse DEAF1 Polyclonal Antibody | anti-DEAF1 antibody

DEAF1 (SPN, ZMYND5, Deformed Epidermal Autoregulatory Factor 1 Homolog, Nuclear DEAF-1-related Transcriptional Regulator, Suppressin, Zinc Finger MYND Domain-containing Protein 5) (FITC)

Cat. Num. AAA6375955

• Gene Names: DEAF1; SPN; NUDR; MRD24; ZMYND5
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DEAF1; Polyclonal Antibody; DEAF1 (SPN; ZMYND5; Deformed Epidermal Autoregulatory Factor 1 Homolog; Nuclear DEAF-1-related Transcriptional Regulator; Suppressin; Zinc Finger MYND Domain-containing Protein 5) (FITC); anti-DEAF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DEAF1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 2737

• Applicable Applications for anti-DEAF1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full-length human DEAF1, aa1-565 (NP_066288.2).
• Immunogen Sequence: MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAGGEAEEPVLSRDEDSEEDADSEAERETPRVTAVAVMAAEPGHMDMGAEALPGPDEAAAAAAFAEVTTVTVANVGAAADNVFTTSVANAASISGHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKNRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGATVQEASVQPPCRASHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPPTPTKAAPPALVNGLELSEPRSWLYLEEMVNSLLNTAQQLKTLFEQAKHASTYREAATNQAKIHADAERKEQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHICGQSAAVTVQADEVHVAESVMEKVTV
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DEAF1 rabbit polyclonal antibody. Western Blot analysis of DEAF1 expression in mouse spleen.)

Western Blot (WB)

(DEAF1 rabbit polyclonal antibody. Western Blot analysis of DEAF1 expression in Hela NE.)

Western Blot (WB)

(Western Blot analysis of DEAF1 expression in transfected 293T cell line by DEAF1 polyclonal antibody. Lane 1: DEAF1 transfected lysate (59.3kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-DEAF1 antibody

Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure and skeletal patterning. Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm.

Product Categories/Family for anti-DEAF1 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 38016944
• NCBI GeneID: 10522
• NCBI Accession #: NM_021008.2
• NCBI GenBank Nucleotide #: NM_021008.2
• UniProt Accession #: O75398
• NCBI Official Full Name: Homo sapiens DEAF1 transcription factor (DEAF1), mRNA
• NCBI Official Synonym Full Names: DEAF1 transcription factor
• NCBI Official Symbol: DEAF1
• NCBI Official Synonym Symbols: SPN; NUDR; MRD24; ZMYND5
• NCBI Protein Information: deformed epidermal autoregulatory factor 1 homolog
• UniProt Protein Name: Deformed epidermal autoregulatory factor 1 homolog
• Protein Family: Deformed epidermal autoregulatory factor
• UniProt Gene Name: DEAF1
• UniProt Synonym Gene Names: SPN; ZMYND5; NUDR
• UniProt Entry Name: DEAF1_HUMAN

NCBI Description

This gene encodes a zinc finger domain-containing protein that functions as a regulator of transcription. The encoded proteins binds to its own promoter as well as to that of several target genes. Activity of this protein is important in the regulation of embryonic development. Mutations in this gene have been found in individuals with autosomal dominant cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

DEAF1: a transcription factor that binds to its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE). Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase.

Protein type: Transcription, coactivator/corepressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; extracellular region; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; regulation of mammary gland epithelial cell proliferation; anatomical structure morphogenesis; behavioral fear response; embryonic skeletal development; positive regulation of transcription, DNA-dependent; neural tube closure; visual learning; negative regulation of transcription, DNA-dependent; germ cell development

Disease: Mental Retardation, Autosomal Dominant 24