Rabbit DEAF1 Polyclonal Antibody | anti-DEAF1 antibody

DEAF1 Antibody

Cat. Num. AAA9631042

• Gene Names: DEAF1; SPN; NUDR; MRD24; ZMYND5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DEAF1; Polyclonal Antibody; DEAF1 Antibody; Deaf1; DEAF1 transcription factor; DEAF1_HUMAN; Deformed epidermal autoregulatory factor 1 homolog; Nuclear DEA1 related transcriptional regulator; Nuclear DEAF-1-related transcriptional regulator; NUDR; SPN; Suppressin; Zinc finger MYND domain containing protein 5; Zinc finger MYND domain-containing protein 5; ZMYND5; anti-DEAF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: DEAF1 Antibody detects endogenous levels of DEAF1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-DEAF1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human DEAF1.
• Conjugation: Unconjugated
• Post Translational Modifications: May be phosphorylated by DNA-PK complex in a DNA independent manner (in vitro).
• Subunit Structure: Homodimer. Isoform 1 and isoform 4 may form a heterodimer. Interacts with LMO2 and CLIM2 (By similarity). Interacts with LMO4; LMO4 blocks export from nucleus (By similarity). May interact with the corepressors NCOR1 and NCRO2. Identified in a complex with the XRCC5 and XRCC6 heterodimer. Interacts (via the SAND domain) with the DNA-PK complex subunit XRCC6; the interaction is direct and may be inhibited by DNA-binding.
• Tissue Specificity: Expressed in various tissues and cells such as in peripheral mononuclear cells and hormone-secreting pituitary cells. Expression in pancreatic lymph nodes of patients with type 1 diabetes is 20 times higher than in healthy controls. Highly expressed in fetal and adult brain.
• Function: Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure and skeletal patterning. Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm. Transcriptional activator of EIF4G3.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-DEAF1 antibody

Nucleus; Cytoplasm; Primary; Secreted; Secreted in some cell types

NCBI and Uniprot Product Information

• NCBI GI #: 38016945
• NCBI GeneID: 10522
• NCBI Accession #: NP_066288.2
• NCBI GenBank Nucleotide #: NM_021008.3
• UniProt Accession #: O75398; O15152; O75399; O75510; O75511; O75512; O75513; Q9UET1; A8K1F8; A8K5R8; C7T5V5
• Molecular Weight: 51,277 Da
• NCBI Official Full Name: deformed epidermal autoregulatory factor 1 homolog isoform a
• NCBI Official Synonym Full Names: DEAF1 transcription factor
• NCBI Official Symbol: DEAF1
• NCBI Official Synonym Symbols: SPN; NUDR; MRD24; ZMYND5
• NCBI Protein Information: deformed epidermal autoregulatory factor 1 homolog; suppressin; zinc finger MYND domain-containing protein 5; nuclear DEAF-1-related transcriptional regulator
• UniProt Protein Name: Deformed epidermal autoregulatory factor 1 homolog
• Protein Family: Deformed epidermal autoregulatory factor
• UniProt Gene Name: DEAF1
• UniProt Synonym Gene Names: SPN; ZMYND5; NUDR
• UniProt Entry Name: DEAF1_HUMAN

NCBI Description

This gene encodes a zinc finger domain-containing protein that functions as a regulator of transcription. The encoded proteins binds to its own promoter as well as to that of several target genes. Activity of this protein is important in the regulation of embryonic development. Mutations in this gene have been found in individuals with autosomal dominant mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

DEAF1: a transcription factor that binds to its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE). Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase.

Protein type: Transcription, coactivator/corepressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; extracellular region; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; regulation of mammary gland epithelial cell proliferation; anatomical structure morphogenesis; behavioral fear response; embryonic skeletal development; positive regulation of transcription, DNA-dependent; neural tube closure; visual learning; negative regulation of transcription, DNA-dependent; germ cell development

Disease: Mental Retardation, Autosomal Dominant 24