Rabbit anti-Human DDX3Y Polyclonal Antibody | anti-DDX3Y antibody

DDX3Y (DBY, ATP-dependent RNA Helicase DDX3Y, DEAD Box Protein 3, Y-chromosomal) (HRP)

Cat. Num. AAA6375890

• Gene Names: DDX3Y; DBY
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DDX3Y; Polyclonal Antibody; DDX3Y (DBY; ATP-dependent RNA Helicase DDX3Y; DEAD Box Protein 3; Y-chromosomal) (HRP); anti-DDX3Y antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DDX3Y.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 660

• Applicable Applications for anti-DDX3Y antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human DDX3Y, aa1-660 (NP_004651.2).
• Immunogen Sequence: MSHVVVKNDPELDQQLANLDLNSEKQSGGASTASKGRYIPPHLRNREASKGFHDKDSSGWSCSKDKDAYSSFGSRDSRGKPGYFSERGSGSRGRFDDRGRSDYDGIGNRERPGFGRFERSGHSRWCDKSVEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGSNCPPHIENFSDIDMGEIIMGNIELTRYTRPTPVQKHAIPIIKGKRDLMACAQTGSGKTAAFLLPILSQIYTDGPGEALKAVKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEDLDKRSFLLDILGATGSDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVRHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNEKNMNITKDLLDLLVEAKQEVPSWLENMAYEHHYKGGSRGRSKSNRFSGGFGARDYRQSSGSSSSGFGASRGSSSRSGGGGYGNSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of DDX3Y expression in transfected 293T cell line by DDX3Y polyclonal antibody. Lane 1: DDX3Y transfected lysate (73.2kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-DDX3Y antibody

Probable ATP-dependent RNA helicase. May play a role in spermatogenesis.

Product Categories/Family for anti-DDX3Y antibody

Antibodies; DEAD Box Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13514809
• NCBI GeneID: 8653
• NCBI Accession #: NP_004651.2
• NCBI GenBank Nucleotide #: NM_004660.2
• UniProt Accession #: O15523
• NCBI Official Full Name: ATP-dependent RNA helicase DDX3Y isoform 1
• NCBI Official Synonym Full Names: DEAD-box helicase 3 Y-linked
• NCBI Official Symbol: DDX3Y
• NCBI Official Synonym Symbols: DBY
• NCBI Protein Information: ATP-dependent RNA helicase DDX3Y
• UniProt Protein Name: ATP-dependent RNA helicase DDX3Y
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DDX3Y
• UniProt Synonym Gene Names: DBY
• UniProt Entry Name: DDX3Y_HUMAN

NCBI Description

The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Uniprot Description

DDX3Y: a Y-linked DEAD box protein. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. DBY mutations causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Two alternatively spliced transcripts differ only in the length of the 3' UTR.

Protein type: RNA-binding; EC 3.6.4.13; Helicase

Chromosomal Location of Human Ortholog: Yq11

Cellular Component: membrane; cytoplasm; nucleus

Molecular Function: DNA binding; RNA binding; helicase activity; ATP binding

Biological Process: metabolic process

Disease: Spermatogenic Failure, Y-linked, 2