Rabbit anti-Human DDX3 Polyclonal Antibody | anti-DDX3 antibody

DDX3, CT (ATP-dependent RNA Helicase DDX3X, DEAD Box Protein 3, X-Chromosomal, Helicase-like Protein 2, HLP2, DEAD Box, X Isoform, DDX3X, DBX) (PE)

Cat. Num. AAA6472107

• Gene Names: DDX3X; DBX; DDX3; HLP2; DDX14; CAP-Rf; MRX102
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DDX3; Polyclonal Antibody; CT (ATP-dependent RNA Helicase DDX3X; DEAD Box Protein 3; X-Chromosomal; Helicase-like Protein 2; HLP2; DEAD Box; X Isoform; DDX3X; DBX) (PE); anti-DDX3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DDX3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DDX3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human DDX3 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DDX3 (arrow) using rabbit polyclonal DDX3 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDX3 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(Western blot analysis of DDX3 Antibody (C-term) in Hela cell line lysates (35ug/lane). DDX3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-DDX3 antibody

Antibodies; DEAD Box Proteins

References

1. Ariumi, Y., J. Virol. 81(24), 13922-13926 (2007). 2. Hogbom, M., J. Mol. Biol. 372(1), 150-159 (2007). 3. Franca, R., Proteins 67(4), 1128-1137 (2007). 4. DDX3, Cancer Res. 66(13), 6579-6588 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 87196351
• NCBI GeneID: 1654
• NCBI Accession #: NP_001347
• NCBI GenBank Nucleotide #: NM_001356
• UniProt Accession #: O00571
• Molecular Weight: 73kDa
• NCBI Official Full Name: ATP-dependent RNA helicase DDX3X isoform 1
• NCBI Official Synonym Full Names: DEAD-box helicase 3 X-linked
• NCBI Official Symbol: DDX3X
• NCBI Official Synonym Symbols: DBX; DDX3; HLP2; DDX14; CAP-Rf; MRX102
• NCBI Protein Information: ATP-dependent RNA helicase DDX3X
• UniProt Protein Name: ATP-dependent RNA helicase DDX3X
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX3X
• UniProt Synonym Gene Names: DBX; DDX3; HLP2
• UniProt Entry Name: DDX3X_HUMAN

NCBI Description

The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

DDX3: a multifunctional DEAD box family RNA helicase with diverse cellular functions. DEAD box proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), and are involved in several steps of gene expression, such as transcription, maturation of nuclear and mitochondrial mRNA, mRNA export, translation initiation, and ribosome and spliceosome assembly. DDX3 is required for nuclear export of HIV-1 viral transcripts, possibly in a complex with the viral Rev protein and host cofactor CRM1. DDX3 is required for hepatitis C virus (HCV) RNA replication and its expression is downregulated in hepatitis B virus (HBV) associated hepatocellular carcinoma (HCC). May function as a tumor suppressor protein. Its expression inhibits tumor cell colony formation and increases expression of the cdk inhibitor p21 Waf1/Cip1. Low DDX3 expression has been shown in HCC, and aberrant subcellular localization occurs in many squamous cell carcinomas. Reduced DDX3 expression in cultured cells causes a diminished dependence on serum for cell proliferation and changes in cyclin D1 and p21 Waf1/Cip1 expression. Associates with eIF4F to promote translation of selected mRNAs. Is phosphorylated by the mitotic cyclin dependent kinase, cyclin B/cdc2. This phosphorylation may cause a loss of DDX3 function and a concomitant repression of ribosome biogenesis and translation in mitosis. Involved in TBK1 and IKBKE-dependent IRF3 activation leading to IFN-beta induction. Involved in regulation of apoptosis. May be required for activation of the intrinsic but inhibit activation of the extrinsic apoptotic pathway. Regulated by the cell cycle. Maximally expressed in the cytoplasm during G1/S phase and decreased expression during G2/M phase. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Associates with the outer side of nuclear pore complexes (NPC) and mitochondrial outer membranes. Shuttles between the nucleus and the cytoplasm in an exportin1-dependent manner. Associates with polyadenylated mRNAs in the cytoplasm and the nucleus. Predominantly located in nucleus during G0 phase and in the cytoplasm during G1/S phase. Belongs to the DEAD box helicase family, DDX3 subfamily. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; RNA-binding; EC 3.6.4.13; Translation initiation; Helicase; Cell cycle regulation

Chromosomal Location of Human Ortholog: Xp11.3-p11.23

Cellular Component: eukaryotic translation initiation factor 3 complex; mitochondrial outer membrane; stress granule; cytoplasm; nuclear speck; nucleus

Molecular Function: GTPase activity; CTPase activity; RNA binding; ATPase activity; transcription factor binding; poly(A) binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; translation initiation factor binding; nucleoside-triphosphatase activity; eukaryotic initiation factor 4E binding; mRNA 5'-UTR binding; ribosomal small subunit binding; ATP binding; ATP-dependent RNA helicase activity

Biological Process: stress granule assembly; positive regulation of viral genome replication; transcription, DNA-dependent; viral reproduction; positive regulation of translation; positive regulation of apoptosis; response to virus; positive regulation of caspase activity; negative regulation of caspase activity; positive regulation of cell growth; DNA duplex unwinding; chromosome segregation; positive regulation of translational initiation; induction of apoptosis via death domain receptors; negative regulation of translation; RNA secondary structure unwinding; positive regulation of interferon-beta production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of protein complex assembly; mature ribosome assembly; negative regulation of apoptosis

Disease: Mental Retardation, X-linked 102