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Western Blot (WB) (Western BlotPositive WB detected in: A549 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, PC3 whole cell lysateAll lanes: DDX28 antibody at 6.1ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60 KDaObserved band size: 60 KDa)

Rabbit anti-Human DDX28 Polyclonal Antibody | anti-DDX28 antibody

DDX28 Antibody

Gene Names
DDX28; MDDX28
Reactivity
Human
Applications
ELISA, Western Blot
Purity
>95%, Protein G purified
Synonyms
DDX28; Polyclonal Antibody; DDX28 Antibody; Probable ATP-dependent RNA helicase DDX28; mitochondrial DEAD box protein 28; MDDX28; anti-DDX28 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
540
Applicable Applications for anti-DDX28 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:500-1:5000
Immunogen
Recombinant Human Probable ATP-dependent RNA helicase DDX28 protein (71-206AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: A549 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, PC3 whole cell lysateAll lanes: DDX28 antibody at 6.1ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60 KDaObserved band size: 60 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: A549 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, PC3 whole cell lysateAll lanes: DDX28 antibody at 6.1ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60 KDaObserved band size: 60 KDa)
Related Product Information for anti-DDX28 antibody
Plays an essential role in facilitating the proper assembly of the mitochondrial large ribosomal subunit and its helicase activity is essential for this function (PubMed:25683708, PubMed:25683715). May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity (PubMed:11350955).
Product Categories/Family for anti-DDX28 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,581 Da
NCBI Official Full Name
probable ATP-dependent RNA helicase DDX28
NCBI Official Synonym Full Names
DEAD-box helicase 28
NCBI Official Symbol
DDX28
NCBI Official Synonym Symbols
MDDX28
NCBI Protein Information
probable ATP-dependent RNA helicase DDX28
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX28
UniProt Gene Name
DDX28
UniProt Synonym Gene Names
MDDX28

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an essential role in facilitating the proper assembly of the mitochondrial large ribosomal subunit and its helicase activity is essential for this function (PubMed:25683708, PubMed:25683715). May be involved in RNA processing or transport. Has RNA and Mg2+-dependent ATPase activity (PubMed:11350955).

Research Articles on DDX28

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Product Notes

The DDX28 ddx28 (Catalog #AAA7107611) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDX28 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDX28 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:500-1:5000. Researchers should empirically determine the suitability of the DDX28 ddx28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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