Rabbit anti-Human DDR2 Polyclonal Antibody | anti-DDR2 antibody

DDR2 (Discoidin Domain-containing Receptor 2, Discoidin Domain Receptor 2, Receptor Protein-tyrosine Kinase TKT, Tyrosine-protein Kinase TYRO10, Neurotrophic Tyrosine Kinase, Receptor-related 3, CD167 Antigen-like Family Member B, CD167b, NTRKR3, TKT, TYR

Cat. Num. AAA6472092

• Gene Names: DDR2; TKT; MIG20a; NTRKR3; TYRO10
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: DDR2; Polyclonal Antibody; DDR2 (Discoidin Domain-containing Receptor 2; Discoidin Domain Receptor 2; Receptor Protein-tyrosine Kinase TKT; Tyrosine-protein Kinase TYRO10; Neurotrophic Tyrosine Kinase; Receptor-related 3; CD167 Antigen-like Family Member B; CD167b; NTRKR3; TKT; TYR; anti-DDR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TYRO10.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-DDR2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: His tag recombinant protein of human TYRO10.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TYRO10 (arrow) using rabbit polyclonal TYRO10 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDR2 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(Western blot analysis of TYRO10 Antibody in Jurkat(lane 1), 293(lane 2) and Hela(lane 3) cell line lysates (35ug/lane). TYRO10 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human normal lung with TYRO10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of jurkat cells using TYRO10 Antibody(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-DDR2 antibody

Antibodies; Receptors

References

1. Vogel, W., et al., Mol. Cell 1(1):13-23 (1997). 2. Edelhoff, S., et al., Genomics 25(1):309-311 (1995). 3. Karn, T., et al., Oncogene 8(12):3433-3440 (1993). 4. Abedinia, M., et al., Biochem. Biophys. Res. Commun. 183(3):1159-1166 (1992). 5. Lapsys, N.M., et al., Cytogenet. Cell Genet. 61(4):274-275 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 215273969
• NCBI GeneID: 4921
• UniProt Accession #: Q16832; Q7Z730
• Molecular Weight: 96,736 Da
• NCBI Official Full Name: Discoidin domain-containing receptor 2
• NCBI Official Synonym Full Names: discoidin domain receptor tyrosine kinase 2
• NCBI Official Symbol: DDR2
• NCBI Official Synonym Symbols: TKT; MIG20a; NTRKR3; TYRO10
• NCBI Protein Information: discoidin domain-containing receptor 2; tyrosylprotein kinase; hydroxyaryl-protein kinase; discoidin domain receptor 2; tyrosine-protein kinase TYRO10; CD167 antigen-like family member B; cell migration-inducing protein 20; migration-inducing gene 16 prot
• UniProt Protein Name: Discoidin domain-containing receptor 2
• Protein Family: Discoidin domain-containing receptor
• UniProt Gene Name: DDR2
• UniProt Synonym Gene Names: NTRKR3; TKT; TYRO10; Discoidin domain receptor 2
• UniProt Entry Name: DDR2_HUMAN

NCBI Description

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

DDR2: This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Protein type: Membrane protein, integral; EC 2.7.10.1; Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; DDR family

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: collagen binding; protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: extracellular matrix organization and biogenesis; ossification; peptidyl-tyrosine phosphorylation; collagen fibril organization; protein amino acid autophosphorylation; signal transduction; positive regulation of osteoblast differentiation; positive regulation of fibroblast proliferation; biomineral formation; positive regulation of protein kinase activity; positive regulation of transcription factor activity; cell adhesion; regulation of bone mineralization

Disease: Spondylometaepiphyseal Dysplasia, Short Limb-hand Type