Rabbit anti-Human, Rat DDR1 Polyclonal Antibody | anti-DDR1 antibody

DDR1, NT (Epithelial Discoidin Domain-containing Receptor 1, Epithelial Discoidin Domain Receptor 1, Tyrosine Kinase DDR, Discoidin Receptor Tyrosine Kinase, Tyrosine-protein Kinase CAK, Cell Adhesion Kinase, TRK E, Protein-tyrosine Kinase 3A, Protein-tyr

Cat. Num. AAA6472067

• Gene Names: DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: DDR1; Polyclonal Antibody; NT (Epithelial Discoidin Domain-containing Receptor 1; Epithelial Discoidin Domain Receptor 1; Tyrosine Kinase DDR; Discoidin Receptor Tyrosine Kinase; Tyrosine-protein Kinase CAK; Cell Adhesion Kinase; TRK E; Protein-tyrosine Kinase 3A; Protein-tyr; anti-DDR1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DDR1. Species Crossreactivity: rat
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DDR1 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 17-47 in the N-terminal region of human DDR1, Swiss/UniProt Accession: Q08345; Q63474; Q03146. Species sequence homology: mouse
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in placenta lysate usingMBS630560. DDR1 (arrow) was detected usingMBS630560. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Western Blot analysis in MCF-7 whole cell lysate (20ug) using MBS630560 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 101kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot analysis in rat brain lysate (20ug) using MBS630560 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 101kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS630560 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS630560 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells using MBS630560 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-DDR1 antibody

Antibodies; Receptors

References

1. Vogel, W., et al., Mol. Cell 1(1):13-23 (1997). 2. Playford, M.P., et al., Genome Res. 6(7):620-627 (1996). 3. Perez, J.L., et al., Oncogene 12(7):1469-1477 (1996). 4. Valent, A., et al., Hum. Genet. 98(1):12-15 (1996). 5. Edelhoff, S., et al., Genomics 25(1):309-311 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 83977450
• NCBI GeneID: 780
• NCBI Accession #: NP_054699
• NCBI GenBank Nucleotide #: NM_013993.2
• UniProt Accession #: Q08345; Q14196; Q16562; Q2L6H3; Q4LE50; Q5ST11; Q5ST12; Q6NSK4; Q9UD35; B5A975; B5A976; B7Z2K0
• Molecular Weight: 71.0kDa (636aa) 70-100KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: epithelial discoidin domain-containing receptor 1 isoform 2
• NCBI Official Synonym Full Names: discoidin domain receptor tyrosine kinase 1
• NCBI Official Symbol: DDR1
• NCBI Official Synonym Symbols: CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
• NCBI Protein Information: epithelial discoidin domain-containing receptor 1
• UniProt Protein Name: Epithelial discoidin domain-containing receptor 1
• Protein Family: Epithelial discoidin domain-containing receptor
• UniProt Gene Name: DDR1
• UniProt Synonym Gene Names: CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; Epithelial discoidin domain receptor 1; MCK-10
• UniProt Entry Name: DDR1_HUMAN

NCBI Description

Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

DDR1: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11. Interacts (via PPxY motif) with WWC1 (via WW domains) in a collagen-regulated manner. Forms a tripartite complex with WWC1 and PRKCZ, but predominantly in the absence of collagen. Interacts (tyrosine phosphorylated) with SHC1. Interacts with SRC. Interacts with MYH9. Interacts with CDH1. Interacts with PTPN11. Interacts with NCK2. Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells. Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; Membrane protein, integral; TK group; DDR family

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: collagen binding; protein binding; metal ion binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: lactation; negative regulation of cell proliferation; extracellular matrix organization and biogenesis; regulation of cell-matrix adhesion; protein amino acid autophosphorylation; smooth muscle cell migration; ear development; regulation of cell growth; cell adhesion; embryo implantation