Rabbit DDIT3 Polyclonal Antibody | anti-DDIT3 antibody

DDIT3 Polyclonal Antibody

Cat. Num. AAA126028

• Gene Names: DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: DDIT3; Polyclonal Antibody; DDIT3 Polyclonal Antibody; CHOP: DDIT3; CEBPZ; CHOP; CHOP10; GADD153; MGC4154; anti-DDIT3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 169

• Applicable Applications for anti-DDIT3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human DDIT3
• Calculated Molecular Weight: 19kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using DDIT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 20s.)

Related Product Information for anti-DDIT3 antibody

DDIT3 was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (1). DDIT3 expression is induced by certain cellular stresses including starvation and the induced DDIT3 suppresses cell cycle progression from G1 to S phase (2). Later it was shown that, during ER stress, the level of DDIT3 expression is elevated and DDIT3 functions to mediate programmed cell death (3). Studies also found that DDIT3 mediates the activation of GADD34 and Ero1-Lalpha expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2alpha thereby stimulating protein synthesis. Ero1-Lalpha promotes oxidative stress inside the endoplasmic reticulum (ER) (4). The role of DDIT3 in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4).

Product Categories/Family for anti-DDIT3 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 544022
• NCBI GeneID: 1649
• UniProt Accession #: P35638; F8VS99
• Molecular Weight: 169
• NCBI Official Full Name: DNA damage-inducible transcript 3 protein
• NCBI Official Synonym Full Names: DNA-damage-inducible transcript 3
• NCBI Official Symbol: DDIT3
• NCBI Official Synonym Symbols: CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
• NCBI Protein Information: DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; c/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; growth arrest and DNA damage-inducible protein GADD153
• UniProt Protein Name: DNA damage-inducible transcript 3 protein
• Protein Family: DNA damage-inducible transcript 3 protein
• UniProt Gene Name: DDIT3
• UniProt Synonym Gene Names: CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10
• UniProt Entry Name: DDIT3_HUMAN

NCBI Description

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Uniprot Description

CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.

Protein type: Autophagy; DNA-binding; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 12q13.1-q13.2

Cellular Component: nucleoplasm; late endosome; cytosol; nucleus

Molecular Function: leucine zipper domain binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor activity; transcription corepressor activity; transcription factor binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-8 production; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; cell cycle arrest; ER overload response; regulation of transcription in response to stress; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; unfolded protein response; negative regulation of transcription factor activity; mRNA transcription from RNA polymerase II promoter; blood vessel maturation; response to unfolded protein; response to starvation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; cell redox homeostasis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; inhibition of CREB transcription factor; negative regulation of myoblast differentiation; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus

Disease: Myxoid Liposarcoma