Western Blot (WB)
(Western blot analysis of DDAH2 expression in rat lung extract (lane 1), mouse lung extract (lane 2) and human placenta extract (lane 3). DDAH2 at 27KD was detected using rabbit anti- DDAH2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit DDAH2 Polyclonal Antibody | anti-DDAH2 antibody
Anti-DDAH2 Antibody
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human
Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Western Blot (WB)
(Western blot analysis of DDAH2 expression in rat lung extract (lane 1), mouse lung extract (lane 2) and human placenta extract (lane 3). DDAH2 at 27KD was detected using rabbit anti- DDAH2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of DDAH2 expression in rat lung extract (lane 1), mouse lung extract (lane 2) and human placenta extract (lane 3). DDAH2 at 27KD was detected using rabbit anti- DDAH2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Immunohistochemistry (IHC)
(DDAH2 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- DDAH2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(DDAH2 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- DDAH2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(DDAH2 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- DDAH2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(DDAH2 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- DDAH2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Background: DDAH2 is known as dimethylarginine dimethylaminohydrolase 2 which is mapped to 6p21.3 by radiation hybrid and FISH analysis. This gene encodes a dimethylarginine dimethylaminohydrolase. DDAH2 functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants.
2. Smith, C. L., Birdsey, G. M., Anthony, S., Arrigoni, F. I., Leiper, J. M., Vallance, P. Dimethylarginine dimethylaminohydrolase activity modulates ADMA levels, VEGF expression, and cell phenotype. Biochem. Biophys. Res. Commun. 308: 984-989, 2003.
3. Tran, C. T. L., Fox, M. F., Vallance, P., Leiper, J. M. Chromosomal localization, gene structure, and expression pattern of DDAH1: comparison with DDAH2 and implications for evolutionary origins. Genomics 68: 101-105, 2000.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants. [provided by RefSeq, Dec 2014]
Uniprot Description
DDAH2: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family.
Protein type: Apoptosis; Hydrolase; EC 3.5.3.18
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: centrosome; cytoplasm; cytosol
Molecular Function: catalytic activity; dimethylargininase activity; protein binding
Biological Process: citrulline metabolic process; negative regulation of apoptosis; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; positive regulation of nitric oxide biosynthetic process; regulation of nitric-oxide synthase activity
Research Articles on DDAH2
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Product Notes
The DDAH2 ddah2 (Catalog #AAA178501) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DDAH2 Antibody reacts with Human, Mouse, Rat No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's DDAH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Mouse, Rat Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human Tested Species:In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Researchers should empirically determine the suitability of the DDAH2 ddah2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDAH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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