Rabbit anti-Human DCTN2 Polyclonal Antibody | anti-DCTN2 antibody

DCTN2, ID (DCTN2, DCTN50, Dynactin subunit 2, 50kD dynein-associated polypeptide, Dynactin complex 50kD subunit, p50 dynamitin) (FITC)

Cat. Num. AAA6291214

• Gene Names: DCTN2; RBP50; DCTN50; HEL-S-77; DYNAMITIN
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: DCTN2; Polyclonal Antibody; ID (DCTN2; DCTN50; Dynactin subunit 2; 50kD dynein-associated polypeptide; Dynactin complex 50kD subunit; p50 dynamitin) (FITC); p50 dynamitin; anti-DCTN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DCTN2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: DCTN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-219 amino acids from the Central region of human DCTN2.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of DCTN2 (arrow) using rabbit polyclonal DCTN2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DCTN2 gene.)

Immunohistochemistry (IHC)

(DCTN2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DCTN2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-DCTN2 antibody

This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.

Product Categories/Family for anti-DCTN2 antibody

Antibodies; Proteins

References

Jacquot, G., et al. J. Biol. Chem. 285(30):23019-23031(2010). Inoue, M., et al. Genes Cells 13(8):905-914(2008). Maier, K.C., et al. Traffic 9(4):481-491(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007). Camargo, L.M., et al. Mol. Psychiatry 12(1):74-86(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 5453629
• NCBI GeneID: 10540
• NCBI Accession #: NP_006391.1
• NCBI GenBank Nucleotide #: NM_006400.4
• UniProt Accession #: Q13561
• NCBI Official Full Name: dynactin subunit 2 isoform 1
• NCBI Official Synonym Full Names: dynactin subunit 2
• NCBI Official Symbol: DCTN2
• NCBI Official Synonym Symbols: RBP50; DCTN50; HEL-S-77; DYNAMITIN
• NCBI Protein Information: dynactin subunit 2
• UniProt Protein Name: Dynactin subunit 2
• Protein Family: Dynactin
• UniProt Gene Name: DCTN2
• UniProt Synonym Gene Names: DCTN50; DCTN-50
• UniProt Entry Name: DCTN2_HUMAN

NCBI Description

This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

dynactin 2: Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. Belongs to the dynactin subunit 2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytoskeletal; Microtubule-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: kinetochore; dynein complex; centrosome; microtubule; dynactin complex; growth cone; membrane; cytoplasm; cytosol; vesicle

Molecular Function: protein binding; spectrin binding; motor activity

Biological Process: mitosis; cell proliferation; mitotic spindle organization and biogenesis; metabolic process; organelle organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; mitotic cell cycle; G2/M transition of mitotic cell cycle; melanosome transport