Rabbit anti-Human, Mouse DCN Polyclonal Antibody | anti-DCN antibody

DCN, ID (DCN, Bone Proteoglycan II, CSCD, DSPG2, PG40, PGII, PGS2, Small Leucine Rich Protein 1B, SLRR1B) (MaxLight 650)

Cat. Num. AAA6472160

• Gene Names: DCN; CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: DCN; Polyclonal Antibody; ID (DCN; Bone Proteoglycan II; CSCD; DSPG2; PG40; PGII; PGS2; Small Leucine Rich Protein 1B; SLRR1B) (MaxLight 650); anti-DCN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DCN. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-DCN antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human DCN (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DCN antibody (Center) in mouse heart tissue lysates (35ug/lane). DCN (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of DCN (arrow) using rabbit polyclonal DCN Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DCN gene.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with DCN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DCN Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-DCN antibody

Antibodies; Carbohydrates; Glycoproteins

References

1. Goldoni, S., J. Cell Biol. 185(4), 743-754 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503271
• NCBI GeneID: 1634
• NCBI Accession #: NP_001911
• NCBI GenBank Nucleotide #: NM_001920.4
• UniProt Accession #: P07585; Q9P0Z0; Q9P0Z1; Q9Y5N8; Q9Y5N9
• Molecular Weight: 38.6kDa (350aa)
• NCBI Official Full Name: decorin isoform a preproprotein
• NCBI Official Synonym Full Names: decorin
• NCBI Official Symbol: DCN
• NCBI Official Synonym Symbols: CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
• NCBI Protein Information: decorin
• UniProt Protein Name: Decorin
• Protein Family: Decorin
• UniProt Gene Name: DCN
• UniProt Synonym Gene Names: SLRR1B
• UniProt Entry Name: PGS2_HUMAN

NCBI Description

This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients. [provided by RefSeq, Nov 2015]

Uniprot Description

DCN: May affect the rate of fibrils formation. Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD). Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; lysosomal lumen; Golgi lumen; extracellular region; collagen type VI

Molecular Function: collagen binding; glycosaminoglycan binding; extracellular matrix binding; protein N-terminus binding

Biological Process: skeletal muscle development; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; wound healing; glycosaminoglycan metabolic process; pathogenesis; response to lipopolysaccharide; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; extracellular matrix disassembly; organ morphogenesis; response to mechanical stimulus; carbohydrate metabolic process; chondroitin sulfate catabolic process; kidney development; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; aging; placenta development

Disease: Corneal Dystrophy, Congenital Stromal