Rabbit anti-Human, Mouse DCK Polyclonal Antibody | anti-DCK antibody

DCK, CT (DCK, Deoxycytidine kinase) (APC)

Cat. Num. AAA6291080

• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, Immunoprecipitation
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: DCK; Polyclonal Antibody; CT (DCK; Deoxycytidine kinase) (APC); Deoxycytidine kinase; anti-DCK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-DCK antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178~207 amino acids from the C-terminal region of human DCK.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(DCK Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DCK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of DCK Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Western Blot (WB)

(The anti-DCK Pab is used in Western blot to detect DCK in mouse intestine tissue lysate.)

Immunoprecipitation (IP)

(Deoxycytidine kinase(DCK) immunoprecipitated from H460cells with 7.5ug (microgram) of the dCK antibody (AP7087b) using the Pierce classic mammalian IP kit (#45217) reagent as described as manufacturer instructions (lane 1, 3) and Current Protocols in Cell Biology, 1998, 7.2.1-7.2.21. Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1:700 dilution of DCK antibody (AP7087b). Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit). No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago.)

Related Product Information for anti-DCK antibody

Deoxycytidine kinase is responsible for the phosphorylation of several deoxyribonucleosides and their analogs. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. It is the rate limiting enzyme in the activation of many important anticancer and retroviral drugs and its activity is often decreased in cells that are resistant to cytosine arabinoside.

Product Categories/Family for anti-DCK antibody

Antibodies; Protein Kinases

References

Chottiner, E. G., et al. Proc. Nat. Acad. Sci. 88: 1531-1535 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 118447
• NCBI GeneID: 1633
• UniProt Accession #: P27707; Q5TZY7; Q6FI11; B2R8V6
• Molecular Weight: 260
• NCBI Official Full Name: Deoxycytidine kinase
• NCBI Official Synonym Full Names: deoxycytidine kinase
• NCBI Official Symbol: DCK
• NCBI Protein Information: deoxycytidine kinase; deoxynucleoside kinase
• UniProt Protein Name: Deoxycytidine kinase
• Protein Family: Deoxycytidine kinase
• UniProt Gene Name: DCK
• UniProt Synonym Gene Names: dCK
• UniProt Entry Name: DCK_HUMAN

NCBI Description

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008]

Uniprot Description

DCK: Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Belongs to the DCK/DGK family.

Protein type: Kinase, other; Nucleotide Metabolism - purine; EC 2.7.1.74; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 4q13.3-q21.1

Cellular Component: cytosol; nucleus

Molecular Function: protein homodimerization activity; nucleoside kinase activity; deoxycytidine kinase activity; drug binding; ATP binding

Biological Process: pyrimidine base metabolic process; pyrimidine nucleotide metabolic process; nucleotide biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; phosphorylation; purine salvage; purine base metabolic process; deoxyribonucleoside monophosphate biosynthetic process