Western Blot (WB)
((0.2ug/ml) staining of COS7 cell lysate transfected with full length recombinant human DCDC2 (A) and untransfected control COS7 cells (B). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat DCDC2 Polyclonal Antibody | anti-DCDC2 antibody
Goat anti-DCDC2 Antibody
Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in select neurons. Recommended concentration, 5-10ug/ml. An anonymous customer found positive results in IHC on Rat retinal tissue. An anonymous customer found positive results in IHC on Rat dorsal root ganglion.
Western Blot: Full length recombinant human DCDC2 expressed in COS7 (Lane A), non-transfectant COS7 (lane B). Approx 52kDa band observed (calculated MW of 52.8kDa according to NP_057440.1). Data kindly provided by University of Connecticut, USA. Recommmended concentration 0.1-0.3ug/ml. An anonymous customer found positive results in WB on Mouse brain tissue.
Western Blot (WB)
((0.2ug/ml) staining of COS7 cell lysate transfected with full length recombinant human DCDC2 (A) and untransfected control COS7 cells (B). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.2ug/ml) staining of COS7 cell lysate transfected with full length recombinant human DCDC2 (A) and untransfected control COS7 cells (B). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a doublecortin domain-containing family member. The doublecortin domain has been demonstrated to bind tubulin and enhance microtubule polymerization. This family member is thought to function in neuronal migration where it may affect the signaling of primary cilia. Mutations in this gene have been associated with reading disability (RD) type 2, also referred to as developmental dyslexia. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2013]
Uniprot Description
DCDC2: May be involved in neuronal migration during development of the cerebral neocortex. Defects in DCDC2 may be a cause of susceptibility to dyslexia type 2 (DYX2); also known as specific reading disability type 2. Dyslexia is a relatively common, complex cognitive disorder that affects 5% to 10% of school-aged children. The disorder is characterized by an impairment of reading performance despite adequate motivational, educational and intellectual opportunities and in the absence of sensory or neurological disability. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 6p22.1
Cellular Component: axoneme; cytoplasm; cytoskeleton; nucleus
Molecular Function: protein binding
Biological Process: cell projection organization and biogenesis; cellular defense response; neuron migration; positive regulation of smoothened signaling pathway; regulation of Wnt receptor signaling pathway; sensory perception of sound
Disease: Deafness, Autosomal Recessive 66; Nephronophthisis 19
Research Articles on DCDC2
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Product Notes
The DCDC2 dcdc2 (Catalog #AAA420508) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DCDC2 Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's DCDC2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in select neurons. Recommended concentration, 5-10ug/ml. An anonymous customer found positive results in IHC on Rat retinal tissue. An anonymous customer found positive results in IHC on Rat dorsal root ganglion. Western Blot: Full length recombinant human DCDC2 expressed in COS7 (Lane A), non-transfectant COS7 (lane B). Approx 52kDa band observed (calculated MW of 52.8kDa according to NP_057440.1). Data kindly provided by University of Connecticut, USA. Recommmended concentration 0.1-0.3ug/ml. An anonymous customer found positive results in WB on Mouse brain tissue. Researchers should empirically determine the suitability of the DCDC2 dcdc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DGEKANKDAE QKED. It is sometimes possible for the material contained within the vial of "DCDC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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