Rabbit anti-Human DCC Polyclonal Antibody | anti-DCC antibody

Rabbit anti DCC Polyclonal Antibody

Cat. Num. AAA460378

• Gene Names: DCC; CRC18; CRCR1; MRMV1; IGDCC1; NTN1R1
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: DCC; Polyclonal Antibody; Rabbit anti DCC Polyclonal Antibody; Deleted in colorectal carcinoma; CRC18; Tumor suppressor protein DCC; anti-DCC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes ~160 kDa of human DCC protein. It also reacts with mouse and rat. The other species are not tested.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 23

• Applicable Applications for anti-DCC antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide corresponding to the C-terminus (within 1380-1447aa) of Human DCC protein.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-DCC antibody

Deleted in colorectal cancer (DCC) was originally identified as a putative tumor suppressor gene that is lost in more than 70% of colorectal cancers. The gene has also been deleted in several other types of cancer. The DCC protein is a type I transmembrane glycoprotein that belongs to the immunoglobulin (Ig) superfamily. The extracellular domain is composed of four Ig-like domains and six fibronectin type III repeats. Native DCC is found in three isoforms. Two forms, a long and a short isoform, are produced from the same gene but have different initiation sites. The third isoform, produced by alternative splicing, is expressed only in embryonic tissue. Mouse DCC extracellular domain shares 97% and 99% homology with human and rat DCC extracellular domains, respectively. In adults, DCC is highly expressed in the brain but is also expressed at very low levels in multiple normal tissues. In the embyro, high levels of expression are detected in the brain and neural tube. DCC functions as a receptor or a component of a receptor for netrins and mediates the effects of netrins on commissural axons. Netrins are chemoattractants responsible for the guidance of commissural axons at the midline and of motor axons to the their target muscles. DCC induces apoptosis in the absence of ligand binding, blocks apoptosis when engaged by netrin 1, and also acts as a caspase substrate.

References

Fearon, E. R., et al. Identification of a chromosome 18q gene that is altered in colorectal cancers. Science 247 (4938), 49-59 (1990)

NCBI and Uniprot Product Information

• NCBI GI #: 1326437
• NCBI GeneID: 1630
• Molecular Weight: 158,457 Da
• NCBI Official Full Name: DCC, partial
• NCBI Official Synonym Full Names: DCC netrin 1 receptor
• NCBI Official Symbol: DCC
• NCBI Official Synonym Symbols: CRC18; CRCR1; MRMV1; IGDCC1; NTN1R1
• NCBI Protein Information: netrin receptor DCC
• UniProt Protein Name: Netrin receptor DCC
• Protein Family: DCC family protein
• UniProt Gene Name: DCC
• UniProt Synonym Gene Names: IGDCC1

NCBI Description

This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma. [provided by RefSeq, Oct 2009]

Uniprot Description

DCC: Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene. Defects in DCC are the cause of mirror movements type 1 (MRMV1). A disorder characterized by contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities. Belongs to the immunoglobulin superfamily. DCC family.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Receptor, misc.; Tumor suppressor

Chromosomal Location of Human Ortholog: 18q21.2

Cellular Component: cytosol; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity

Biological Process: apoptosis; axon guidance; axonogenesis; negative regulation of collateral sprouting

Disease: Colorectal Cancer; Esophageal Cancer; Mirror Movements 1