Rabbit anti-Human DAXX Polyclonal Antibody | anti-DAXX antibody

DAXX Antibody (S495)

Cat. Num. AAA9217151

• Gene Names: DAXX; DAP6; EAP1; BING2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: DAXX; Polyclonal Antibody; DAXX Antibody (S495); Death domain-associated protein 6; Daxx; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein; BING2; DAP6; anti-DAXX antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This DAXX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-502 amino acids from human DAXX.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 740

• Applicable Applications for anti-DAXX antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:1000
• Subtitle: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Cytoplasm. Nucleus, nucleoplasm. Nucleus, PML body. Nucleus, nucleolus. Chromosome, centromere. Note=Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli. Colocalizes with histone H3.3, ATRX, HIRA and ASF1A at PML-nuclear bodies. Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres Detected in cytoplasmic punctate structures. Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress. Colocalizes with RASSF1 in the nucleus. Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures Isoform gamma: Nucleus. Note=Diffuse nuclear distribution pattern and no comparable dot-like accumulation of isoform 1.
• Tissue Location: Ubiquitous.
• Antigen Region: 473-502 aa
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-DAXX Antibody (S495) at 1:1000 dilution + K562 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 81 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-DAXX antibody

Description: DAXX is a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, this protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, this protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination.

Background: Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activatiopn of PAX3 and ETS1 through direct protein-protein interactions. Modulates PAX5 activity; the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Acts as histone chaperone that facilitates deposition of histone H3. 3. Acts as targeting component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3. 3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3. 3-containing nucleosomes. Does not affect the ATPase activity of ATRX but alleviates its transcription repression activity. Upopn neuronal activation asociates with regulatory elements of selected immediate early genes where it promotes deposition of histone H3. 3 which may be linked to transcriptional induction of these genes. Required for the recruitment of histone H3. 3:H4 dimers to PML-nuclear bodies (PML- NBs); the process is independent of ATRX and facilitated by ASF1A; PML-NBs are suggested to function as regulatory sites for the incorporation of newly synthesized histone H3. 3 into chromatin. In case of overexpression of centromeric histone variant CENPA (as found in various tumors) is involved in its mislocalization to chromosomes; the ectopic localization involves a heterotypic tetramer containing CENPA, and histones H3. 3 and H4 and decreases binding of CTCF to chromatin. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Shows restriction activity towards human cytomegalovirus (HCMV).

Product Categories/Family for anti-DAXX antibody

Primary Antibodies; Antibody Collections; Cell Biology; Microbiology; Amyotrophic Lateral Sclerosis (ALS)

References

Cantrell, S.R., et al., J. Virol. 79 (12):7792-7802 (2005). Greger, J.G., et al., J. Virol. 79 (8):4610-4618 (2005). Chang, C.C., et al., J. Biol. Chem. 280 (11):10164-10173 (2005). Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101 (33):12130-12135 (2004). Gostissa, M., et al., J. Biol. Chem. 279 (46):48013-48023 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 215422388
• NCBI GeneID: 1616
• NCBI Accession #: NP_001135441.1
• NCBI GenBank Nucleotide #: NM_001141969.1
• UniProt Accession #: Q9UER7; O14747; O15141; O15208; Q5STK9; Q9BWI3; B4E1I3; F5ANJ6; F5ANJ7; F5H082
• Molecular Weight: 81373 Da
• NCBI Official Full Name: death domain-associated protein 6 isoform a
• NCBI Official Synonym Full Names: death-domain associated protein
• NCBI Official Symbol: DAXX
• NCBI Official Synonym Symbols: DAP6; EAP1; BING2
• NCBI Protein Information: death domain-associated protein 6
• UniProt Protein Name: Death domain-associated protein 6
• Protein Family: Death domain-associated protein
• UniProt Gene Name: DAXX
• UniProt Synonym Gene Names: BING2; DAP6; hDaxx; EAP1
• UniProt Entry Name: DAXX_HUMAN

NCBI Description

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

DAXX: a transcriptional co-regulatory protein. Proposed to mediate activation of the JNK pathway and apoptosis via ASK1 in response to signaling from FAS and TGF-betaR2. Glucose deprivation activates the ASK1-SEK1-JNK1-HIPK1 pathway, relocalizing Daxx from the nucleus to the cytoplasm, where Daxx binds to ASK1, and subsequently leads to ASK1 oligomerization. Interaction with HSP27 may prevent interaction with TGF-betaR2 and ASK1 and block DAXX-mediated apoptosis. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Two alternatively spliced isoforms have been described.

Protein type: Nucleolus; Apoptosis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell cortex; chromosome, pericentric region; cytoplasm; cytosol; microtubule; neuron projection; nucleolus; nucleoplasm; nucleus; PML body; XY body

Molecular Function: androgen receptor binding; enzyme binding; heat shock protein binding; histone binding; p53 binding; protein binding; protein homodimerization activity; protein kinase activator activity; protein kinase binding; protein N-terminus binding; receptor signaling protein activity; transcription corepressor activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: activation of JNK activity; androgen receptor signaling pathway; apoptosis; chromatin remodeling; induction of apoptosis via death domain receptors; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nucleosome assembly; PML body organization and biogenesis; positive regulation of apoptosis; positive regulation of histone phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase activity; regulation of protein ubiquitination; regulation of transcription, DNA-dependent; response to metal ion; transcription, DNA-dependent; viral reproduction