Rabbit anti-Human DARS Polyclonal Antibody | anti-DARS antibody

DARS, NT (DARS, Aspartate-tRNA ligase, cytoplasmic, Aspartyl-tRNA synthetase, Cell proliferation-inducing gene 40 protein) (HRP)

Cat. Num. AAA6290852

• Gene Names: DARS; HBSL; aspRS
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: DARS; Polyclonal Antibody; NT (DARS; Aspartate-tRNA ligase; cytoplasmic; Aspartyl-tRNA synthetase; Cell proliferation-inducing gene 40 protein) (HRP); Aspartate--tRNA ligase; Cell proliferation-inducing gene 40 protein; anti-DARS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DARS antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IF: 1:10-50; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: DARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from the N-terminal region of human DARS.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(DARS Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the DARS antibody detected the DARS protein (arrow).)

Western Blot (WB)

(Western blot analysis of DARS (arrow) using rabbit polyclonal DARS Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.)

Immunohistochemistry (IHC)

(DARS Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DARS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of DARS Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of DARS Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-DARS antibody

Antibodies; Enzymes; Synthase

References

Wu, C., et al. Proteomics 7(11):1775-1785(2007). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Tu, L.C., et al. Mol. Cell Proteomics 6(4):575-588(2007). Ling, C., et al. J. Biol. Chem. 280(41):34755-34763(2005). Bonnefond, L., et al. Biochemistry 44(12):4805-4816(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 45439306
• NCBI GeneID: 1615
• NCBI Accession #: NP_001340.2
• NCBI GenBank Nucleotide #: NM_001349.3
• UniProt Accession #: P14868; Q2TNI3; Q32Q69; Q53HV4; Q53YC5; Q68CR9; Q9BW52; A8K3J2; D3DP77
• Molecular Weight: 45,771 Da
• NCBI Official Full Name: aspartate--tRNA ligase, cytoplasmic isoform 1
• NCBI Official Synonym Full Names: aspartyl-tRNA synthetase
• NCBI Official Symbol: DARS
• NCBI Official Synonym Symbols: HBSL; aspRS
• NCBI Protein Information: aspartate--tRNA ligase, cytoplasmic; aspartate tRNA ligase 1, cytoplasmic; aspartyl-tRNA synthetase, cytoplasmic; cell proliferation-inducing gene 40 protein
• UniProt Protein Name: Aspartate--tRNA ligase, cytoplasmic
• UniProt Gene Name: DARS
• UniProt Synonym Gene Names: AspRS
• UniProt Entry Name: SYDC_HUMAN

NCBI Description

This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

DARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: Translation; EC 6.1.1.12; Ligase

Chromosomal Location of Human Ortholog: 2q21.3

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; aspartate-tRNA ligase activity; ATP binding; aminoacylase activity

Biological Process: tRNA aminoacylation for protein translation; translation; aspartyl-tRNA aminoacylation; protein complex assembly; gene expression

Disease: Hypomyelination With Brainstem And Spinal Cord Involvement And Leg Spasticity