Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using DAPP1 (Phospho-Tyr139) Antibody. The picture on the right is blocked with the phospho peptide.)
Rabbit anti-Human, Mouse DAPP1 Polyclonal Antibody | anti-DAPP1 antibody
DAPP1 (Phospho-Tyr139) Antibody
Gene Names
DAPP1; BAM32
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
DAPP1; Polyclonal Antibody; DAPP1 (Phospho-Tyr139) Antibody; 3' phosphoinositide-interacting SH2 domain containing protein; B lymphocyte adapter protein BAM32; Bam32; Dual adaptor for phosphotyrosine and 3-phosphoinositides 1; PHISH; dual adapter for phosphotyrosine and 3-phosphoinositides; anti-DAPP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
DAPP1 (Phospho-Tyr139) Antibody detects endogenous levels of DAPP1 only when phosphorylated at Tyr139.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
280
Applicable Applications for anti-DAPP1 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:5000
IF: 1:100~1:500
ELISA: 1:5000
Target Modification
Phospho
Modification Sites
H:Y139; M:Y139
Immunogen
The antiserum was produced against synthesized peptide derived from human DAPP1 around the phosphorylation site of Tyr139.
Immunogen Range
105-154
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using DAPP1 (Phospho-Tyr139) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using DAPP1 (Phospho-Tyr139) Antibody. The picture on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 2', using DAPP1 (Phospho-Tyr139) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 2', using DAPP1 (Phospho-Tyr139) Antibody. The lane on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32 kDa
NCBI Official Full Name
dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide isoform 2
NCBI Official Synonym Full Names
dual adaptor of phosphotyrosine and 3-phosphoinositides 1
NCBI Official Symbol
DAPP1
NCBI Official Synonym Symbols
BAM32
NCBI Protein Information
dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
UniProt Protein Name
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
UniProt Gene Name
DAPP1
UniProt Synonym Gene Names
BAM32; hDAPP1
Uniprot Description
May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
Research Articles on DAPP1
Similar Products
Product Notes
The DAPP1 dapp1 (Catalog #AAA9502737) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAPP1 (Phospho-Tyr139) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DAPP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the DAPP1 dapp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAPP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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