Rabbit anti-Mouse DAP3 Polyclonal Antibody | anti-DAP3 antibody

DAP3 (Death Associated Protein3)

Cat. Num. AAA611344

• Gene Names: DAP3; DAP-3; MRPS29; MRP-S29; bMRP-10; MGC126058; MGC126059; DKFZp686G12159; DAP3
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: DAP3; Polyclonal Antibody; DAP3 (Death Associated Protein3); Anti -DAP3 (Death Associated Protein3); anti-DAP3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse DAP3 (Death Associated Protein3)
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-DAP3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-2ug/ml; Whole cell lysate from Jurkat cells can be used as positive control and an approximatley 45kD band should be detected.
• Immunogen: Two synthetic peptides corresponding to amino acids 44-52 and 358-371 of mouse DAP-3 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of 10 ug of total cell lysate from Jurkat cells with MBS611344 at 1 ug/ml.)

Related Product Information for anti-DAP3 antibody

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively (2-3). Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction (3).

Product Categories/Family for anti-DAP3 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 55662994
• NCBI GeneID: 7818
• UniProt Accession #: P51398; Q13044; Q68CT7; Q96Q20
• Molecular Weight: 45,566 Da
• NCBI Official Full Name: death associated protein 3
• NCBI Official Synonym Full Names: death associated protein 3
• NCBI Official Symbol: DAP3
• NCBI Official Synonym Symbols: DAP-3; MRPS29; MRP-S29; bMRP-10; MGC126058; MGC126059; DKFZp686G12159; DAP3
• NCBI Protein Information: 28S ribosomal protein S29, mitochondrial; S29mt; death-associated protein 3; mitochondrial 28S ribosomal protein S29; ionizing radiation resistance conferring protein
• UniProt Protein Name: 28S ribosomal protein S29, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: DAP3
• UniProt Synonym Gene Names: MRPS29
• UniProt Entry Name: RT29_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq]

Uniprot Description

DAP3: Involved in mediating interferon-gamma-induced cell death. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Mitochondrial; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: nucleoplasm; small ribosomal subunit; mitochondrion; mitochondrial inner membrane; mitochondrial ribosome; nucleolus; nucleus

Molecular Function: protein binding

Biological Process: apoptotic mitochondrial changes; mitochondrial translation; organelle organization and biogenesis