Western Blot (WB)
((1ug/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35ug protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat DAP3 Polyclonal Antibody | anti-DAP3 antibody
Goat anti-DAP3 Antibody
Western Blot: Approx 40kDa band observed in lysates of cell line HeLa (calculated MW of 45.6kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. The band fades in HeLa after si-RNA-mediated knock-down (including the fainter shoulder band at 37kDa). Recommended concentration: 1-3ug/ml.
Immunocytochemistry: Mitochondrial staining of HeLa after in situ denaturation of the target protein using 3-4M guanidinium thiocyanate (patent pending). The subcellular localisation is in agreement with Morgan et al. , Biochem Biophys Res Commun.2001 Jan 12;280(1): 177-81. (PMID: 11162496) and Harada et al. , Apoptosis.2010 Oct;15(10): 1247-55. (PMID: 20563667). The signal goes down upon knockdown of the DAP3 expression. Recommended concentration: 3-10ug/ml.
Western Blot (WB)
((1ug/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35ug protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35ug protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(Staining (3ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (3ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing DAP3 (RC223182) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing DAP3 (RC223182) and probed (mock transfection in first lane), tested by Origene.)
Immunofluorescence (IF)
((0.5ug/ml) staining of guanidinium thiocyanate-treated HeLa before (left) and after (right) si-RNA-mediated DAP3 knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain.)
Immunofluorescence (IF)
((0.5ug/ml) staining of guanidinium thiocyanate-treated HeLa before (left) and after (right) si-RNA-mediated DAP3 knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain.)
NCBI and Uniprot Product Information
NCBI Description
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Dec 2010]
Uniprot Description
DAP3: Involved in mediating interferon-gamma-induced cell death. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor co-regulator; Apoptosis; Mitochondrial
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: mitochondrial inner membrane; mitochondrial small ribosomal subunit; mitochondrion; nucleoplasm
Molecular Function: protein binding; structural constituent of ribosome
Research Articles on DAP3
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Product Notes
The DAP3 dap3 (Catalog #AAA420985) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DAP3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's DAP3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 40kDa band observed in lysates of cell line HeLa (calculated MW of 45.6kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. The band fades in HeLa after si-RNA-mediated knock-down (including the fainter shoulder band at 37kDa). Recommended concentration: 1-3ug/ml. Immunocytochemistry: Mitochondrial staining of HeLa after in situ denaturation of the target protein using 3-4M guanidinium thiocyanate (patent pending). The subcellular localisation is in agreement with Morgan et al., Biochem Biophys Res Commun.2001 Jan 12;280(1): 177-81. (PMID: 11162496) and Harada et al., Apoptosis.2010 Oct;15(10): 1247-55. (PMID: 20563667). The signal goes down upon knockdown of the DAP3 expression. Recommended concentration: 3-10ug/ml. Researchers should empirically determine the suitability of the DAP3 dap3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NPSLLERHCA YL. It is sometimes possible for the material contained within the vial of "DAP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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