Rabbit DAI Polyclonal Antibody | anti-DAI antibody

RABBIT ANTI DAI

Cat. Num. AAA223548

• Gene Names: ZBP1; DAI; DLM1; DLM-1; C20orf183
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: DAI; Polyclonal Antibody; RABBIT ANTI DAI; anti-DAI antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This product recognizes human DAI, also known as Z-DNA Binding Protein (ZBP1), a 46kDa DNA binding protein belonging to a family of proteins that contain a Za domain which specifically binds to the left-handed Z-DNA and Z-RNA. ; ; Initial studies suggested that DAI may be involved in processes such host response to neoplasia. Additional research has indicated that the cytosolic DAI can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of DAI to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK). ; ; DAI is highly expressed in lymphatic tissues such as lymph nodes, leukocytes, tonsil, bone marrow and spleen. Expression at a lower level is also seen in thymus, lung and liver. It has been demonstrated that multiple isoforms of DAI exist.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 428

• Applicable Applications for anti-DAI antibody: ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)*
• Application Notes: Western Blot: This item detects a band of approximately 46 kDa in mouse small intestine tissue lysate; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml; Application Note: Reduced and denatured conditions
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide corresponding to an 18 amino acid sequence from near the carboxy terminus of human ZBP1.; Buffer Solution
• Antiserum Preparation: Antisera to human DAI were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of mouse small intestine lysate probed with Rabbit anti Human DAI (MBS223498) at 0.5(A), 1(B) and 2(C))

Testing Data #2

(Formalin fixed, paraffin embedded human small intestine stained with Rabbit anti Human DAI (MBS223498) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-DAI antibody

This product recognizes human DAI, also known as Z-DNA Binding Protein (ZBP1), a 46kDa DNA binding protein belonging to a family of proteins that contain a Za domain which specifically binds to the left-handed Z-DNA and Z-RNA. Initial studies suggested that DAI may be involved in processes such host response to neoplasia. Additional research has indicated that the cytosolic DAI can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of DAI to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK). DAI is highly expressed in lymphatic tissues such as lymph nodes, leukocytes, tonsil, bone marrow and spleen. Expression at a lower level is also seen in thymus, lung and liver. It has been demonstrated that multiple isoforms of DAI exist.

NCBI and Uniprot Product Information

• NCBI GI #: 238550208
• NCBI GeneID: 81030
• NCBI Accession #: NP_001153889.1
• NCBI GenBank Nucleotide #: NM_001160417.1
• UniProt Accession #: Q9H171; Q5JY39; Q9BYW4; A2A2F7; B3KVA1; F5GYT1
• Molecular Weight: 38,220 Da
• NCBI Official Full Name: Z-DNA-binding protein 1 isoform b
• NCBI Official Synonym Full Names: Z-DNA binding protein 1
• NCBI Official Symbol: ZBP1
• NCBI Official Synonym Symbols: DAI; DLM1; DLM-1; C20orf183
• NCBI Protein Information: Z-DNA-binding protein 1; DNA-dependent activator of IFN-regulatory factors; DNA-dependent activator of IRFs; DNA-dependent activator of interferon regulatory factors; tumor stroma and activated macrophage protein DLM-1
• UniProt Protein Name: Z-DNA-binding protein 1
• UniProt Gene Name: ZBP1
• UniProt Synonym Gene Names: C20orf183; DLM1
• UniProt Entry Name: ZBP1_HUMAN

NCBI Description

This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

ZBP1: Participates in the detection by the host's innate immune system of DNA from viral, bacterial or even host origin. Plays a role in host defense against tumors and pathogens. Acts as a cytoplasmic DNA sensor which, when activated, induces the recruitment of TBK1 and IRF3 to its C-terminal region and activates the downstream interferon regulatory factor (IRF) and NF-kappa B transcription factors, leading to type-I interferon production. ZBP1-induced NF-kappaB activation probably involves the recruitment of the RHIM containing kinases RIPK1 and RIPK3. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: double-stranded RNA adenosine deaminase activity; protein binding; DNA binding; left-handed Z-DNA binding; RNA binding

Biological Process: metabolic process; positive regulation of interferon type I production; innate immune response