Rabbit anti-Human DAAM1 Polyclonal Antibody | anti-DAAM1 antibody

DAAM1, NT (Disheveled-associated Activator Of Morphogenesis 1, DAAM1, KIAA0666) (Biotin)

Cat. Num. AAA6471813

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: DAAM1; Polyclonal Antibody; NT (Disheveled-associated Activator Of Morphogenesis 1; KIAA0666) (Biotin); anti-DAAM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DAAM1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DAAM1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human DAAM1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DAAM1 (arrow) using rabbit polyclonal DAAM1 Antibody(Human N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DAAM1 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with DAAM1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-DAAM1 antibody

Antibodies; Actin Related Proteins

References

1. Liu, W., Proc. Natl. Acad. Sci. USA 105(1), 210-215 (2008). 2. Yamashita, M., Genes Cells 12(11), 1255-1265 (2007). 3. Lu, J., J. Mol. Biol. 369(5), 1258-1269 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 21071077
• NCBI GeneID: 23002
• NCBI Accession #: NP_055807
• NCBI GenBank Nucleotide #: NM_014992
• UniProt Accession #: Q9Y4D1
• Molecular Weight: 123kDa
• NCBI Official Full Name: disheveled-associated activator of morphogenesis 1 isoform 1
• NCBI Official Synonym Full Names: dishevelled associated activator of morphogenesis 1
• NCBI Official Symbol: DAAM1
• NCBI Protein Information: disheveled-associated activator of morphogenesis 1
• UniProt Protein Name: Disheveled-associated activator of morphogenesis 1
• Protein Family: Disheveled-associated activator of morphogenesis
• UniProt Gene Name: DAAM1
• UniProt Synonym Gene Names: KIAA0666
• UniProt Entry Name: DAAM1_HUMAN

NCBI Description

Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2012]

Uniprot Description

DAAM1: Binds to disheveled (Dvl) and Rho, and mediates Wnt- induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Belongs to the formin homology family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: membrane; cytoplasm; stress fiber; plasma membrane; cytosol

Molecular Function: identical protein binding; protein binding; Rho GTPase binding; actin binding

Biological Process: actin cytoskeleton organization and biogenesis