Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Rabbit D-Dopachrome Tautomerase (DDT) Polyclonal Antibody | anti-DDT antibody
Biotin-Linked Antibody to D-Dopachrome Tautomerase (DDT)
Reactivity
Homo sapiens (Human)
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
Purity
Antigen-specific Affinity Chromatography.
Synonyms
D-Dopachrome Tautomerase (DDT); Polyclonal Antibody; Biotin-Linked Antibody to D-Dopachrome Tautomerase (DDT); anti-DDT antibody
Host
Rabbit
Reactivity
Homo sapiens (Human)
Clonality
Polyclonal
Purity/Purification
Antigen-specific Affinity Chromatography.
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.46mg/ml (varies by lot)
Applicable Applications for anti-DDT antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (IHC), Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Antibody labeling
Label
Biotin
Traits
Liquid
Immunogen
Recombinant DDT (Pro2~Leu118) expressed in E.coli ( MBS2012104 ).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C for up to 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C for up to 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Product Categories/Family for anti-DDT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
D-dopachrome tautomerase
Protein Family
Uniprot Description
DDT: Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). Belongs to the MIF family.
Protein type: Lyase; EC 4.1.1.84
Chromosomal Location of Human Ortholog: 22q11.23
Cellular Component: cytoplasm
Molecular Function: D-dopachrome decarboxylase activity; dopachrome isomerase activity
Biological Process: melanin biosynthetic process
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Product Notes
The DDT (Catalog #AAA2002881) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Biotin-Linked Antibody to D-Dopachrome Tautomerase (DDT) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's D-Dopachrome Tautomerase (DDT) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (IHC), Immunoprecipitation (IP). . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the DDT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "D-Dopachrome Tautomerase (DDT), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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