D-4 Dopamine receptor IgG fraction Polyclonal Antibody | anti-DRD4 antibody
D-4 Dopamine receptor IgG fraction
Gene Names
DRD4; D4DR
Applications
Immunoblot, Western Blot, Western Blot
Synonyms
D-4 Dopamine receptor IgG fraction; Polyclonal Antibody; D 4 (176-185); D 4 Dopamine Receptor; IgG Fraction of Rabbit Anti-D4 Receptor Sera; anti-DRD4 antibody
Clonality
Polyclonal
Specificity
Polypeptide ::: % Cross Reactivity
D4 Dopamine Receptor (Ac-176-185) ::: 100
D4 Dopamine Receptor ::: 60
D4 Dopamine Receptor (Ac-176-185) ::: 100
D4 Dopamine Receptor ::: 60
Form/Format
Antiserum Specificity
Sequence Length
467
Applicable Applications for anti-DRD4 antibody
Immunocytochemical Staining, Western Immunoblot, Western Blot
Application Notes
Immunocytochemistry: This IgG fraction has been found to stain specific cells in various regions of PLP fixed rat brain sections at a concentration of 5 ug/ml. This includes the glomeruli of the olfactory bulb. staining of cortex layer 4 and dendritic staining in cortex layers 2 and 3. In layer 5 of the cortex apical dendrites of many neurons were stained. In the hippocampus diffuse immunoreactivity was observed in the molecular layer of the dentate gyrus. with fine dots associated with many neurons in the granule cell layer.
Western Blot: Western immunoblots using whole rat brain homogenate resulted in a single wide band being detected at ~48-53 kD at a concentration of 40 ug/ml.
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the rabbit polyclonal antibody after diluting to 40 ug/ml. Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Western Blot: Western immunoblots using whole rat brain homogenate resulted in a single wide band being detected at ~48-53 kD at a concentration of 40 ug/ml.
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the rabbit polyclonal antibody after diluting to 40 ug/ml. Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Related Product Information for anti-DRD4 antibody
The antiserum was raised in a rabbit which was immunized with D4(Ac-176-185) covalently attached onto a carrier protein. and it has been characterized by immunocytochemical. western immunoblot and ELISA techniques. The antiserum and the IgG fraction of the antiserum have been found to be highly specific for this peptide sequence and are suitable for immunocytochemical detection of the D4 dopamine receptor. Rehydrate the lyophilized IgG fraction with 0.1 ml of PBS which contains 10 mg/ml BSA or with additional buffer (such as 10 mg/ml BSA in PBS) for more dilute antibody. Each vial contains 2.78 mg of purified rabbit IgG.
Product Categories/Family for anti-DRD4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,361 Da
NCBI Official Full Name
D(4) dopamine receptor
NCBI Official Synonym Full Names
dopamine receptor D4
NCBI Official Symbol
DRD4
NCBI Official Synonym Symbols
D4DR
NCBI Protein Information
D(4) dopamine receptor; D(4) dopamine receptor; OTTHUMP00000164435; dopamine D4 receptor; D(2C) dopamine receptor; seven transmembrane helix receptor
UniProt Protein Name
D(4) dopamine receptor
Protein Family
UniProt Gene Name
DRD4
UniProt Entry Name
DRD4_HUMAN
NCBI Description
This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats. [provided by RefSeq]
Uniprot Description
Function: This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Subunit structure: Interacts with CLIC6
By similarity and GPRASP1. Ref.9
Subcellular location: Cell membrane; Multi-pass membrane protein.
Polymorphism: The number of repeats of 16 amino acids in the third cytoplasmic loop is highly polymorphic and varies among different alleles. Alleles corresponding in size to a 2 (D4.2), 3 (D4.3), 4 (D4.4), 5 (D4.5), 6 (D4.6), 7 (D4.7) and 9 (D4.9) repeats have been described. The sequence shown is that of allele D4.7. The polymorphic repeat sequence has little influence on DRD4-binding profiles and might not be essential for G protein interaction.
Sequence similarities: Belongs to the G-protein coupled receptor 1 family.
Sequence caution: The sequence AAL58637.1 differs from that shown. Reason: Erroneous gene model prediction.
Research Articles on DRD4
Similar Products
Product Notes
The DRD4 drd4 (Catalog #AAA352075) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's D-4 Dopamine receptor IgG fraction can be used in a range of immunoassay formats including, but not limited to, Immunocytochemical Staining, Western Immunoblot, Western Blot. Immunocytochemistry: This IgG fraction has been found to stain specific cells in various regions of PLP fixed rat brain sections at a concentration of 5 ug/ml. This includes the glomeruli of the olfactory bulb. staining of cortex layer 4 and dendritic staining in cortex layers 2 and 3. In layer 5 of the cortex apical dendrites of many neurons were stained. In the hippocampus diffuse immunoreactivity was observed in the molecular layer of the dentate gyrus. with fine dots associated with many neurons in the granule cell layer. Western Blot: Western immunoblots using whole rat brain homogenate resulted in a single wide band being detected at ~48-53 kD at a concentration of 40 ug/ml. Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer. 2. Wash x 2 with TBS/Tween-20. 3. Apply the rabbit polyclonal antibody after diluting to 40 ug/ml. Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours. 4. Wash x 3 with TBS/Tween-20. 5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques. 6. Wash x 4 for 5 minute/wash with TBS/Tween-20. 7. Develop color using the enhanced DAB reaction. Researchers should empirically determine the suitability of the DRD4 drd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "D-4 Dopamine receptor IgG fraction, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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