Rabbit anti-Human D-3-phosphoglycerate dehydrogenase Polyclonal Antibody | anti-PHGDH antibody

Rabbit anti-human D-3-phosphoglycerate dehydrogenase polyclonal Antibody, FITC

Cat. Num. AAA719516

• Gene Names: PHGDH; NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
• Reactivity: Human
• Applications: ELISA
• Purity: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Synonyms: D-3-phosphoglycerate dehydrogenase; Polyclonal Antibody; Rabbit anti-human D-3-phosphoglycerate dehydrogenase polyclonal Antibody; FITC; PGDH3 PHGDH 3-PGDH; anti-PHGDH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Sequence Length: 533

• Applicable Applications for anti-PHGDH antibody: ELISA (EIA)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: FITC
• Immunogen: Recombinant human D-3-phosphoglycerate dehydrogenase protein
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-16222
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blot All lanes: D-3-phosphoglycerate dehydrogenase ntibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 59 kDa Observed band size: 59kDa)

NCBI and Uniprot Product Information

• NCBI GI #: 23308577
• NCBI GeneID: 26227
• NCBI Accession #: NP_006614.2
• NCBI GenBank Nucleotide #: NM_006623.3
• UniProt Accession #: O43175; Q5SZU3; Q9BQ01; B2RD08
• Molecular Weight: 56,651 Da
• NCBI Official Full Name: D-3-phosphoglycerate dehydrogenase
• NCBI Official Synonym Full Names: phosphoglycerate dehydrogenase
• NCBI Official Symbol: PHGDH
• NCBI Official Synonym Symbols: NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
• NCBI Protein Information: D-3-phosphoglycerate dehydrogenase
• UniProt Protein Name: D-3-phosphoglycerate dehydrogenase
• Protein Family: D-3-phosphoglycerate dehydrogenase
• UniProt Gene Name: PHGDH
• UniProt Synonym Gene Names: PGDH3; 3-PGDH
• UniProt Entry Name: SERA_HUMAN

NCBI Description

This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]

Uniprot Description

PHGDH: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation, and seizures. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Protein type: Cell development/differentiation; Amino Acid Metabolism - glycine, serine and threonine; EC 1.1.1.95; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: cytosol; myelin sheath

Molecular Function: electron carrier activity; NAD binding; phosphoglycerate dehydrogenase activity

Biological Process: amino acid biosynthetic process; brain development; gamma-aminobutyric acid metabolic process; glial cell development; glutamine metabolic process; glycine metabolic process; L-serine biosynthetic process; neural tube development; neurite development; regulation of gene expression; serine family amino acid biosynthetic process; spinal cord development; taurine metabolic process; threonine metabolic process

Disease: Neu-laxova Syndrome 1; Phosphoglycerate Dehydrogenase Deficiency