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Western Blot (WB)
(Anti-KRT14 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:53 kDa Observed band size:53 kDa)
Rabbit anti-Mouse Cytokeratin 14/KRT14 Polyclonal Antibody | anti-KRT14 antibody
Anti-Cytokeratin 14/KRT14 Antibody, Rabbit Polyclonal
Gene Names
Krt14; K14; Krt1-14; AI626930; Krt-1.14
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
Cytokeratin 14/KRT14; Polyclonal Antibody; Anti-Cytokeratin 14/KRT14 Antibody; Rabbit Polyclonal; KRT14 Antibody; Rabbit PAb; Antigen Affinity Purified; keratin 14; type I; Anti-AI626930 Antibody; Anti-K14 Antibody; Anti-Krt-1.14 Antibody; Anti-Krt1-14 Antibody; anti-KRT14 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse KRT14
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-KRT14 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:2000-1:10,000
IP: 0.5-2uL/mg of lysate
IHC-P: 1:2000-1:10,000
IP: 0.5-2uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the center region of the Mouse KRT14
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse KRT14, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-KRT14 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:53 kDa Observed band size:53 kDa)
Western Blot (WB)
(Anti-KRT14 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:53 kDa Observed band size:53 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse KRT14 in mouse skin with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse KRT14 in mouse skin with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse KRT14 in mouse prostate with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse KRT14 in mouse prostate with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(KRT14 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 2 uL anti-KRT14 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-KRT14 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 53 kDa Observed band size :53 kDa)
Immunoprecipitation (IP)
(KRT14 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 2 uL anti-KRT14 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-KRT14 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 53 kDa Observed band size :53 kDa)
Related Product Information for anti-KRT14 antibody
Cytokeratin 14, also known as Keratin 14 and K14, is a member of the keratin family. Cytokeratin 14 is a type I keratin. It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Cytokeratin 14 is mainly expressed in the basal layer. It is also strongly expressed in the outer root sheath of anagen follicles. Cytokeratin 14 and keratin 5 may have a role in the maintenance of cell proliferation potential in the basal layer of stratified epithelia, modulating phosphatidylinositol 3-kinase/Akt-mediated cell proliferation and/or Notch1-dependent cell differentiation. Cytokeratin 14 defect prevents it from working effectively with keratin 5 and interfering with the assembly of the keratin intermediate filament network. A disruption in this network makes keratinocytes fragile and prone to rupture. Minor trauma to the skin, such as rubbing or scratching, can cause these cells to break down, resulting in the formation of painful, fluid-filled blisters. Mutations in the K14 gene are also responsible for Naegeli-Franceschetti-Jadassohn syndrome and Dermatopathia Pigmentosa Reticularis.
References
Coulombe PA, et al., 1991, Cell. 66(6): 1301-11.Schweizer J, et al., 2006, 174(2): 169-74.Lugassy J, et al., 2006, Am J Hum Genet. 79(4): 724-30.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,867 Da
NCBI Official Full Name
keratin, type I cytoskeletal 14
NCBI Official Synonym Full Names
keratin 14
NCBI Official Symbol
Krt14
NCBI Official Synonym Symbols
K14; Krt1-14; AI626930; Krt-1.14
NCBI Protein Information
keratin, type I cytoskeletal 14; CK-14; keratin-14; cytokeratin 14; cytokeratin-14; keratin complex 1, acidic, gene 14; epidermolysis bullosa simplex, Dowling-Meara, Koebner
UniProt Protein Name
Keratin, type I cytoskeletal 14
Protein Family
UniProt Gene Name
Krt14
UniProt Synonym Gene Names
Krt1-14; CK-14; K14
UniProt Entry Name
K1C14_MOUSE
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Product Notes
The KRT14 krt14 (Catalog #AAA8103384) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytokeratin 14/KRT14 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 14/KRT14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:2000-1:10,000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the KRT14 krt14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 14/KRT14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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