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Western Blot (WB) (Western Blot: Sample: Lane1: Rat Liver Tissue; Lane2: Mouse Liver Tissue.)

Rabbit anti-Rat Cytochrome P450 1A1 Polyclonal Antibody | anti-CYP1A1 antibody

Polyclonal Antibody to Cytochrome P450 1A1 (CYP1A1)

Gene Names
Cyp1a1; AHH; AHRR; CP11; CYP1; Cyp45c; P1-450; P450-C; P450DX; Cypc45c; P-450MC
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Cytochrome P450 1A1; Polyclonal Antibody; Polyclonal Antibody to Cytochrome P450 1A1 (CYP1A1); anti-CYP1A1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CYP1A1. It has beenselected for its ability to recognize CYP1A1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
518
Applicable Applications for anti-CYP1A1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Fragment
CYP1A1 (Ser251~His521)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Mouse, Rat
Conjugate
No Conjugate
Immunogen
Recombinant CYP1A1 (Ser251~His521) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2070824
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Rat Liver Tissue; Lane2: Mouse Liver Tissue.)

Western Blot (WB) (Western Blot: Sample: Lane1: Rat Liver Tissue; Lane2: Mouse Liver Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant CYP1A1, Rat.)

Western Blot (WB) (Western Blot: Sample: Recombinant CYP1A1, Rat.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Liver Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Liver Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Pancreas Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Pancreas Tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,393 Da
NCBI Official Full Name
cytochrome P450 1A1
NCBI Official Synonym Full Names
cytochrome P450, family 1, subfamily a, polypeptide 1
NCBI Official Symbol
Cyp1a1
NCBI Official Synonym Symbols
AHH; AHRR; CP11; CYP1; Cyp45c; P1-450; P450-C; P450DX; Cypc45c; P-450MC
NCBI Protein Information
cytochrome P450 1A1
UniProt Protein Name
Cytochrome P450 1A1
Protein Family
UniProt Gene Name
Cyp1a1
UniProt Synonym Gene Names
Cyp1a-1; CYP1A1

NCBI Description

monooxygenase that plays a role in dioxin metabolism and detoxification which is 3-methylcholanthrene-inducible [RGD, Feb 2006]

Uniprot Description

CYP1A1: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics

Protein type: Amino Acid Metabolism - tryptophan; Cofactor and Vitamin Metabolism - retinol; EC 1.14.14.1; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: cytoplasm; endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrial membrane; mitochondrion

Molecular Function: catalytic activity; demethylase activity; enzyme binding; flavonoid 3'-monooxygenase activity; heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on diphenols and related substances as donors; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; protein binding; steroid hydroxylase activity

Biological Process: 9-cis-retinoic acid biosynthetic process; aging; amine metabolic process; camera-type eye development; cell proliferation; coumarin metabolic process; developmental process; dibenzo-p-dioxin catabolic process; dibenzo-p-dioxin metabolic process; drug metabolic process; flavonoid metabolic process; gut development; heterocycle metabolic process; hydrogen peroxide biosynthetic process; insecticide metabolic process; liver development; maternal process involved in parturition; porphyrin metabolic process; response to antibiotic; response to arsenic; response to drug; response to food; response to herbicide; response to hyperoxia; response to hypoxia; response to iron(III) ion; response to lipopolysaccharide; response to nematode; response to organic cyclic compound; response to organic substance; response to toxin; response to virus; response to vitamin A; response to wounding; steroid metabolic process; toxin metabolic process

Research Articles on CYP1A1

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Product Notes

The CYP1A1 cyp1a1 (Catalog #AAA2027559) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cytochrome P450 1A1 (CYP1A1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 1A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CYP1A1 cyp1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 1A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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