Western Blot (WB)
(Figure 1. Western blot analysis of CYP51A1/CYP51 using anti-CYP51A1/CYP51 antibody (MBS1753636).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: monkey COS-7 whole cell lysatesLane 2: human A431 whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: mouse liver tissue lysatesLane 5: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CYP51A1/CYP51 antigen affinity purified polyclonal antibody (Catalog # MBS1753636) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for CYP51A1/CYP51 at approximately 57KD. The expected band size for CYP51A1/CYP51 is at 57KD. )
Rabbit CYP51A1/CYP51 Polyclonal Antibody | anti-CYP51A1 antibody
Anti-CYP51A1/CYP51 Antibody
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of CYP51A1/CYP51 using anti-CYP51A1/CYP51 antibody (MBS1753636).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: monkey COS-7 whole cell lysatesLane 2: human A431 whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: mouse liver tissue lysatesLane 5: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CYP51A1/CYP51 antigen affinity purified polyclonal antibody (Catalog # MBS1753636) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for CYP51A1/CYP51 at approximately 57KD. The expected band size for CYP51A1/CYP51 is at 57KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of CYP51A1/CYP51 using anti-CYP51A1/CYP51 antibody (MBS1753636).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: monkey COS-7 whole cell lysatesLane 2: human A431 whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: mouse liver tissue lysatesLane 5: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CYP51A1/CYP51 antigen affinity purified polyclonal antibody (Catalog # MBS1753636) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for CYP51A1/CYP51 at approximately 57KD. The expected band size for CYP51A1/CYP51 is at 57KD. )
Immunofluorescence (IF)
(Figure 2. IF analysis of CYP51A1/CYP51 using anti- CYP51A1/CYP51 antibody (MBS1753636).CYP51A1/CYP51 was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- CYP51A1/CYP51 Antibody (MBS1753636) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Immunofluorescence (IF)
(Figure 2. IF analysis of CYP51A1/CYP51 using anti- CYP51A1/CYP51 antibody (MBS1753636).CYP51A1/CYP51 was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- CYP51A1/CYP51 Antibody (MBS1753636) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of HepG2 cells using anti-CYP51A1/CYP51 antibody (MBS1753636).Overlay histogram showing HepG2 cells stained with MBS1753636 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP51A1/CYP51 Antibody (MBS1753636, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of HepG2 cells using anti-CYP51A1/CYP51 antibody (MBS1753636).Overlay histogram showing HepG2 cells stained with MBS1753636 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP51A1/CYP51 Antibody (MBS1753636, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Gillespie, R. L., O'Sullivan, J., Ashworth, J., Bhaskar, S., Williams, S., Biswas, S., Kehdi, E., Ramsden, S. C., Clayton-Smith, J., Black, G. C., Lloyd, I. C. Personalized diagnosis and management of congenital cataract by next-generation sequencing. Ophthalmology 121: 2124-2137, 2014.
3. Hubler, Z., Allimuthu, D., Bederman, I., Elitt, M. S., Madhavan, M., Allan, K. C., Shick, H. E., Garrison, E., Karl, M. T., Factor, D. C., Nevin, Z. S., Sax, J. L. {and 12 others}: Accumulation of 8,9-unsaturated sterols drives oligodendrocyte formation and remyelination. Nature 560: 372-376, 2018.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Uniprot Description
CYP51A1: Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; EC 1.14.13.70; Lipid Metabolism - steroid biosynthesis; Oxidoreductase
Chromosomal Location of Human Ortholog: 7q21.2
Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane
Molecular Function: sterol 14-demethylase activity; iron ion binding; heme binding
Biological Process: xenobiotic metabolic process; sterol metabolic process; cholesterol biosynthetic process; steroid biosynthetic process; cholesterol biosynthetic process via 24,25-dihydrolanosterol
Research Articles on CYP51A1
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Product Notes
The CYP51A1 cyp51a1 (Catalog #AAA1753636) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CYP51A1/CYP51 Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CYP51A1/CYP51 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Monkey| ICC/IF: 5ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the CYP51A1 cyp51a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYP51A1/CYP51, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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