Rabbit anti-Human CYP3A5 Polyclonal Antibody | anti-CYP3A5 antibody

CYP3A5, ID (Cytochrome P450 3A5, CYPIIIA5, Cytochrome P450-PCN3, Cytochrome P450 HLp2) (MaxLight 550)

Cat. Num. AAA6471554

• Gene Names: CYP3A5; CP35; PCN3; CYPIIIA5; P450PCN3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CYP3A5; Polyclonal Antibody; ID (Cytochrome P450 3A5; CYPIIIA5; Cytochrome P450-PCN3; Cytochrome P450 HLp2) (MaxLight 550); anti-CYP3A5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP3A5.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-CYP3A5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CYP3A5 (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CYP3A5 Antibody (Center) in 293 cell line lysates (35ug/lane). CYP3A5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GPD1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CYP3A5 Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CYP3A5 antibody

Antibodies; Enzymes; Cytochrome

References

1. Zencir, S., Z. Naturforsch., C, J. Biosci. 63(9-10), 780-784 (2008). 2. Nelson, D.R., Pharmacogenetics 14(1), 1-18 (2004). 3. Murray, G.I., FEBS Lett. 364(1), 79-82 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4503231
• NCBI GeneID: 1577
• NCBI Accession #: NP_000768
• NCBI GenBank Nucleotide #: NM_000777
• UniProt Accession #: P20815
• Molecular Weight: 57kDa
• NCBI Official Full Name: cytochrome P450 3A5 isoform 1
• NCBI Official Synonym Full Names: cytochrome P450 family 3 subfamily A member 5
• NCBI Official Symbol: CYP3A5
• NCBI Official Synonym Symbols: CP35; PCN3; CYPIIIA5; P450PCN3
• NCBI Protein Information: cytochrome P450 3A5
• UniProt Protein Name: Cytochrome P450 3A5
• UniProt Gene Name: CYP3A5
• UniProt Entry Name: CP3A5_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein metabolizes drugs as well as the steroid hormones testosterone and progesterone. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Two pseudogenes of this gene have been identified within this cluster on chromosome 7. Expression of this gene is widely variable among populations, and a single nucleotide polymorphism that affects transcript splicing has been associated with susceptibility to hypertensions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor: Heme group

By similarity.

Subcellular location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction: P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Miscellaneous: Chimeric transcripts, characterized by CYP3A43 exon 1 joined at canonical splice sites to distinct sets of CYP3A5 exons, have been detected. All are possibly produced by trans-splicing. The chimeric transcripts exist in 2 different combinations: CYP3A43 exon 1 joined in frame to CYP3A5 exon 11-13 and CYP3A43 exon 1 joined in frame to CYP3A5 exon 12-13. All chimeric transcripts are expressed at very low levels in the liver (Ref.11).

Sequence similarities: Belongs to the cytochrome P450 family.