Goat CYP19 / Aromatase Polyclonal Antibody | anti-CYP19 antibody

Goat Polyclonal to Human CYP19 / Aromatase

Cat. Num. AAA242564

• Gene Names: CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CYP19 / Aromatase; Polyclonal Antibody; Goat Polyclonal to Human CYP19 / Aromatase; Anti-CYP19 / Aromatase Antibody (Internal) IHC-plus; CYP19A1; ARO1; CYAR; CPV1; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; CYP19; Estrogen synthase; Estrogen synthetase; P-450AROM; ARO; Aromatase; Human CYP19; anti-CYP19 antibody

• Host: Goat
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CYP19A1 / Aromatase. This antibody is expected to recognise both reported isoforms (NP_000094.2 and NP_112503.1), which are identical.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 503

• Applicable Applications for anti-CYP19 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (4 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-HDLSLHPDETKN from an internal region of human CYP19A1 / Aromatase (NP_000094.2; NP_112503.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (92%).
• Immunogen Type: Synthetic peptide
• Immunogen: CYP19 / Aromatase antibody was raised against synthetic peptide C-HDLSLHPDETKN from an internal region of human CYP19A1 / Aromatase (NP_000094.2; NP_112503.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (92%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.)

Western Blot (WB)

(Antibody (0.1 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-CYP19 antibody

Aromatase, also known as estrogen synthetase, is a P450 enzyme that catalyzes the formation of aromatic C18 estrogens from C19 androgens. Aromatase is present in many tissues including skin, muscle, brain, adipose, and placenta where it may contribute to sex-specific differences in cellular metabolism.

Product Categories/Family for anti-CYP19 antibody

Family: Cytochrome P450

NCBI and Uniprot Product Information

• NCBI GI #: 13904858
• NCBI GeneID: 1588
• NCBI Accession #: NP_000094.2
• NCBI GenBank Nucleotide #: NM_000103.3
• UniProt Accession #: P11511; Q16731; Q3B764; Q58FA0; Q8IYJ7
• Molecular Weight: 24,518 Da
• NCBI Official Full Name: aromatase
• NCBI Official Synonym Full Names: cytochrome P450, family 19, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP19A1
• NCBI Official Synonym Symbols: ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• NCBI Protein Information: aromatase; estrogen synthase; estrogen synthetase; cytochrome P-450AROM; cytochrome P450 19A1; microsomal monooxygenase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily XIX (aromatization of androgens)
• UniProt Protein Name: Aromatase
• UniProt Gene Name: CYP19A1
• UniProt Synonym Gene Names: ARO1; CYAR; CYP19
• UniProt Entry Name: CP19A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; Lipid Metabolism - androgen and estrogen; EC 1.14.14.14

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum

Molecular Function: electron carrier activity; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding

Biological Process: steroid metabolic process; xenobiotic metabolic process; androgen metabolic process; steroid biosynthetic process; sterol metabolic process; estrogen biosynthetic process

Disease: Aromatase Deficiency; Aromatase Excess Syndrome