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Western Blot (WB) (Western blot analysis of Cyclin E2 expression in HeLa cell lysate.)

Rabbit anti-Human, Mouse Cyclin E2 Polyclonal Antibody | anti-CCNE2 antibody

Anti-Cyclin E2 Rabbit pAb

Gene Names
CCNE2; CYCE2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
Cyclin E2; Polyclonal Antibody; Anti-Cyclin E2 Rabbit pAb; CCN E2; CCNE 2; CCNE2 protein; CYC E2; CYCE 2; CyclinE2; G1/S specific cyclin E2; anti-CCNE2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Cyclin E2 Antibody detects endogenous levels of Cyclin E2
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
404
Applicable Applications for anti-CCNE2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC/IF: 1:100-1:500
FC/FACS: 1:20
Immunogen
A synthesized peptide derived from human Cyclin E2
Subcellular Location
Cytoplasm; Cytoskeleton
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of Cyclin E2 expression in HeLa cell lysate.)

Western Blot (WB) (Western blot analysis of Cyclin E2 expression in HeLa cell lysate.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cyclin E2 Antibody.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cyclin E2 Antibody.)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells, using Cyclin E2 Antibody.)

Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells, using Cyclin E2 Antibody.)
Related Product Information for anti-CCNE2 antibody
Cyclin E1 and cyclin E2 can associate with and activate CDK2. Upon DNA damage, upregulatioctivation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. Essential for the control of the cell cycle at the late G1 and early S phase.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47kDa
NCBI Official Full Name
G1/S-specific cyclin-E2
NCBI Official Synonym Full Names
cyclin E2
NCBI Official Symbol
CCNE2
NCBI Official Synonym Symbols
CYCE2
NCBI Protein Information
G1/S-specific cyclin-E2
UniProt Protein Name
G1/S-specific cyclin-E2
Protein Family
UniProt Gene Name
CCNE2

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq, Jul 2008]

Uniprot Description

Essential for the control of the cell cycle at the late G1 and early S phase.

Research Articles on CCNE2

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Product Notes

The CCNE2 ccne2 (Catalog #AAA4750546) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclin E2 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin E2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC/IF: 1:100-1:500 FC/FACS: 1:20. Researchers should empirically determine the suitability of the CCNE2 ccne2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin E2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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