Western Blot (WB)
(Western blot analysis of Cyclin D1 (pT286) expression in MCF7 EGF-treated (A), rat muscle (B) whole cell lysates.)
Rabbit anti-Human, Rat Cyclin D1 Polyclonal Antibody | anti-CCND1 antibody
Anti-Cyclin D1 (pT286) Phospho Antibody
Western Blot (WB)
(Western blot analysis of Cyclin D1 (pT286) expression in MCF7 EGF-treated (A), rat muscle (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Cyclin D1 (pT286) expression in MCF7 EGF-treated (A), rat muscle (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Cyclin D1 (pT286) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Cyclin D1 (pT286) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.
Protein type: Activator; Nuclear receptor co-regulator; Cell cycle regulation; Oncoprotein
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: nucleoplasm; tight junction; membrane; transcriptional repressor complex; cyclin-dependent protein kinase holoenzyme complex; intracellular; nucleus; cytosol
Molecular Function: protein binding; enzyme binding; histone deacetylase binding; protein complex binding; cyclin-dependent protein kinase regulator activity; transcription factor binding; protein kinase binding; transcription corepressor activity; protein kinase activity
Biological Process: G1 DNA damage checkpoint; fat cell differentiation; lactation; establishment and/or maintenance of chromatin architecture; positive regulation of cyclin-dependent protein kinase activity; negative regulation of Wnt receptor signaling pathway; re-entry into mitotic cell cycle; positive regulation of mammary gland epithelial cell proliferation; negative regulation of epithelial cell differentiation; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; response to magnesium ion; response to vitamin E; Leydig cell differentiation; response to iron ion; response to corticosterone stimulus; response to X-ray; response to drug; Notch signaling pathway; organ regeneration; transcription, DNA-dependent; unfolded protein response; mammary gland epithelial cell proliferation; liver development; response to organic nitrogen; response to ethanol; response to estrogen stimulus; cell division; mitotic cell cycle; positive regulation of protein amino acid phosphorylation; response to calcium ion; response to DNA damage stimulus; G1/S transition of mitotic cell cycle
Disease: Von Hippel-lindau Syndrome; Myeloma, Multiple
Research Articles on CCND1
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Product Notes
The CCND1 ccnd1 (Catalog #AAA8234111) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclin D1 (pT286) Phospho Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin D1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the CCND1 ccnd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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