Rabbit CYCLIN D1 Polyclonal Antibody | anti-CYCLIN D1 antibody

RABBIT ANTI CYCLIN D1

Cat. Num. AAA222202

• Gene Names: CCND1; BCL1; PRAD1; U21B31; D11S287E
• Reactivity: Amphibia (Expected from Sequence), Chimpanzee (Expected from Sequence), Dog (Expected from Sequence), Mouse, Rat, Zebrafish (Expected from Sequence)
• Applications: ELISA, Western Blot
• Synonyms: CYCLIN D1; Polyclonal Antibody; RABBIT ANTI CYCLIN D1; anti-CYCLIN D1 antibody

• Host: Rabbit
• Reactivity: Amphibia (Expected from Sequence), Chimpanzee (Expected from Sequence), Dog (Expected from Sequence), Mouse, Rat, Zebrafish (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item is specific for Cyclin D1, a member of the cyclin family that function as CKD reculators. Cyclin D1 is involved in cell cycle transition and has been shown to interact with the retinoblastoma protein. The unprocessed precursor is 295 amino acids long. It is expressed in abnormally high levels in a number of cancers and is specifically implicated in breast cancer development.
• Form/Format: Serum; Serum - liquid
• Sequence Length: 295

• Applicable Applications for anti-CYCLIN D1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: When used on asynchronous HN30 cell lysates, a band was observed at approximately 34 kDa corresponding to Cyclin D1.; ELISA: Minimum Dilution: 1/2,000; Maximum Dilution: 1/10,000; Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/1,000
• Perservative Stabilisers: 0.01% Sodium Azide (NaN3) ; Preparation
• Immunogen: Full length synthetic polypeptide corresponding to the human gene sequence.; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CYCLIN D1 antibody

MBS222202 is specific for Cyclin D1, a member of the cyclin family that function as CKD reculators. Cyclin D1 is involved in cell cycle transition and has been shown to interact with the retinoblastoma protein. The unprocessed precursor is 295 amino acids long. It is expressed in abnormally high levels in a number of cancers and is specifically implicated in breast cancer development.

NCBI and Uniprot Product Information

• NCBI GI #: 16950655
• NCBI GeneID: 595
• NCBI Accession #: NP_444284.1
• NCBI GenBank Nucleotide #: NM_053056.2
• UniProt Accession #: P24385; Q6LEF0
• Molecular Weight: 33,729 Da
• NCBI Official Full Name: G1/S-specific cyclin-D1
• NCBI Official Synonym Full Names: cyclin D1
• NCBI Official Symbol: CCND1
• NCBI Official Synonym Symbols: BCL1; PRAD1; U21B31; D11S287E
• NCBI Protein Information: G1/S-specific cyclin-D1; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1 oncogene; PRAD1 oncogene
• UniProt Protein Name: G1/S-specific cyclin-D1
• UniProt Gene Name: CCND1
• UniProt Synonym Gene Names: BCL1; PRAD1; BCL-1
• UniProt Entry Name: CCND1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.

Protein type: Oncoprotein; Cell cycle regulation; Nuclear receptor co-regulator; Activator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; tight junction; transcriptional repressor complex; membrane; cyclin-dependent protein kinase holoenzyme complex; intracellular; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; protein complex binding; cyclin-dependent protein kinase regulator activity; transcription factor binding; protein kinase binding; transcription corepressor activity; protein kinase activity

Biological Process: G1 DNA damage checkpoint; fat cell differentiation; lactation; establishment and/or maintenance of chromatin architecture; positive regulation of cyclin-dependent protein kinase activity; re-entry into mitotic cell cycle; negative regulation of Wnt receptor signaling pathway; positive regulation of mammary gland epithelial cell proliferation; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; response to magnesium ion; response to vitamin E; Leydig cell differentiation; response to iron ion; response to X-ray; response to corticosterone stimulus; response to drug; Notch signaling pathway; organ regeneration; transcription, DNA-dependent; unfolded protein response; mammary gland epithelial cell proliferation; liver development; response to organic nitrogen; response to ethanol; response to estrogen stimulus; cell division; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; response to calcium ion; response to DNA damage stimulus; G1/S transition of mitotic cell cycle

Disease: Von Hippel-lindau Syndrome; Myeloma, Multiple