Rabbit Cyclin D1 Polyclonal Antibody | anti-CCND1 antibody

Rabbit anti Cyclin D1 Polyclonal Antibody

Cat. Num. AAA460117

• Gene Names: CCND1; BCL1; PRAD1; U21B31; D11S287E
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: Cyclin D1; Polyclonal Antibody; Rabbit anti Cyclin D1 Polyclonal Antibody; CCND1; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL1; G1/S-specific cyclin D1; anti-CCND1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes 36 kDa of Cyclin D1 Protein. It reacts with human, mouse and rat. The other species are not tested.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 125

• Applicable Applications for anti-CCND1 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide derived from C-terminus of human cyclin D1 protein. This sequence is identical to human, mouse, rat.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-CCND1 antibody

Cyclin family is highly conserved which are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. The increased expression of cyclin D1 can play a critical role in tumor development and in maintenance of the malignant phenotype, such as human neoplasms including mantle cell lymphomas (MCL). Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

References

Tashiro E. Tsuchiya A, Imoto M. Functions of cyclin D1 as an oncognene and regulation of cyclin D1 expression. Cancer Sci, 98 (5): 629-35 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 11414805
• NCBI GeneID: 595
• UniProt Accession #: Q9H014; P24385
• Molecular Weight: 13,986 Da
• NCBI Official Full Name: cyclin D1, partial
• NCBI Official Synonym Full Names: cyclin D1
• NCBI Official Symbol: CCND1
• NCBI Official Synonym Symbols: BCL1; PRAD1; U21B31; D11S287E
• NCBI Protein Information: G1/S-specific cyclin-D1
• UniProt Protein Name: Cyclin D1
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: cyclin D1

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]