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Western Blot (WB) (Western Blot: Sample: Mouse Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Human CCNB2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Human Cyclin B2 Polyclonal Antibody | anti-CCNB2 antibody

Polyclonal Antibody to Cyclin B2 (CCNB2)

Gene Names
CCNB2; HsT17299
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Cyclin B2; Polyclonal Antibody; Polyclonal Antibody to Cyclin B2 (CCNB2); anti-CCNB2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CCNB2. It has beenselected for its ability to recognize CCNB2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
398
Applicable Applications for anti-CCNB2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
CCNB2 (Val58~Ser398)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant CCNB2 (Val58~Ser398) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2070794
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Human CCNB2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Human CCNB2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Human Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P. Samples: Human Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45,282 Da
NCBI Official Full Name
G2/mitotic-specific cyclin-B2
NCBI Official Synonym Full Names
cyclin B2
NCBI Official Symbol
CCNB2
NCBI Official Synonym Symbols
HsT17299
NCBI Protein Information
G2/mitotic-specific cyclin-B2
UniProt Protein Name
G2/mitotic-specific cyclin-B2
UniProt Gene Name
CCNB2

NCBI Description

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNB2: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Belongs to the cyclin family. Cyclin AB subfamily.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: centrosome; cytosol; membrane; microtubule cytoskeleton; nucleus

Molecular Function: cadherin binding; cyclin-dependent protein kinase activity; protein binding

Biological Process: cell division; G2/M transition of mitotic cell cycle; growth; in utero embryonic development; mitotic nuclear envelope disassembly; regulation of cell cycle; T cell homeostasis; thymus development

Research Articles on CCNB2

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Product Notes

The CCNB2 ccnb2 (Catalog #AAA2028332) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cyclin B2 (CCNB2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin B2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the CCNB2 ccnb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin B2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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