Western Blot (WB)
(Cell extract was separated on SDS-PAGE, blotted with 1.5 ug/mL CYBB (NOX2) antibody, and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lane 1: RAW264; Lane 2: RAW264 + Antibody + NOX2 immunizing peptide.)
Rabbit anti-Human CYBB Polyclonal Antibody | anti-CYBB antibody
CYBB Antibody
Gene Names
CYBB; CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
Reactivity
Human
Applications
Western Blot
Purity
The antibody is affinity-purified using the immunizing peptide immobilized on agarose
Synonyms
CYBB; Polyclonal Antibody; CYBB Antibody; Affinity Purified Rabbit CYBB Antibody; Polyclonal CYBB antibody; Anti-CYBB antibody; GP91-1 antibody; GP91PHOX antibody; NOX2 antibody; Chronic Granulomatous Disease antibody; Cytochrome B-245 Beta Polypeptide antibody; GP91-PHOX antibody; CGD antibody; anti-CYBB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human NOX2/CYBB
Purity/Purification
The antibody is affinity-purified using the immunizing peptide immobilized on agarose
Form/Format
Supplied as a solution in 0.01 M Phosphate Buffered Saline, pH 7.4, containing 15 mM Sodium Azide.
Concentration
1.5 mg/ml (varies by lot)
Sequence Length
35
Applicable Applications for anti-CYBB antibody
Western Blot (WB)
Application Notes
WB: 1.5-3.0 ug/ml
Immunogen
CYBB antibody was raised in rabbit using a synthetic peptide corresponding to amino acids 150-166 of human NOX2 the as immunogen.
Preparation and Storage
For continuous use, store at 2-8 deg C for up to one month. For extended storage, freeze in working aliquots.
Western Blot (WB)
(Cell extract was separated on SDS-PAGE, blotted with 1.5 ug/mL CYBB (NOX2) antibody, and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lane 1: RAW264; Lane 2: RAW264 + Antibody + NOX2 immunizing peptide.)
Western Blot (WB)
(Cell extract was separated on SDS-PAGE, blotted with 1.5 ug/mL CYBB (NOX2) antibody, and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lane 1: RAW264; Lane 2: RAW264 + Antibody + NOX2 immunizing peptide.)
Related Product Information for anti-CYBB antibody
CYBB (also known as cytochrome b-245, beta polypeptide, NOX2, CGD, GP91-1, GP91PHOX, p91-PHOX) is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD).
Product Categories/Family for anti-CYBB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CYBB, partial
NCBI Official Synonym Full Names
cytochrome b-245 beta chain
NCBI Official Symbol
CYBB
NCBI Official Synonym Symbols
CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
NCBI Protein Information
cytochrome b-245 heavy chain
Protein Family
NCBI Description
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]
Research Articles on CYBB
Similar Products
Product Notes
The CYBB (Catalog #AAA5316447) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYBB Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CYBB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1.5-3.0 ug/ml. Researchers should empirically determine the suitability of the CYBB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYBB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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